基于指纹图谱与主成分分析相结合的复方虎杖方提取工艺研究

发布时间：2018-05-26 14:46

本文选题：复方虎杖方 + 均匀设计　；参考：《中草药》2017年02期

【摘要】：目的建立指纹图谱与主成分分析(PCA)相结合的提取工艺优化方法,并将其应用于复方虎杖方乙醇提取工艺优化。方法以复方虎杖方为模型药物,采用HPLC法建立指纹图谱,对共有峰峰面积进行PCA,以总因子得分为评价指标,采用U9(93×31)均匀设计法考察提取次数(X1)、乙醇用量(X2)、乙醇体积分数(X3)和提取时间(X4)对提取效果的影响,建立回归模型,确定最佳工艺参数。结果建立的数学模型能够较好地描述效应指标与各因素之间的关系(r=0.997),应用建立的方法优化得到复方虎杖方最适宜醇提工艺为6倍量90%乙醇提取3次,每次1 h。结论所建立的方法简便有效,适合复方虎杖方提取,可以为复方中药提取工艺优化提供参考。
[Abstract]:Objective to establish a method for optimizing the extraction process of fingerprint and principal component analysis (PCA). The method was applied to the optimization of ethanol extraction process of compound tiger stick. Method the compound tiger stick was used as model drug and HPLC method was used to establish fingerprint. The total peak area was PCA, and the total factor score was used as the evaluation index, and U9 (93 x 31) were used. The effect of extraction times (X1), ethanol amount (X2), ethanol volume fraction (X3) and extraction time (X4) on the extraction effect was investigated by uniform design. The regression model was established to determine the optimum process parameters. The mathematical model established can describe the relationship between the effect index and the various factors (r=0.997), and the compound method was used to optimize the compound formula. The most suitable alcohol extraction process of tiger stick is 6 times the amount of 90% ethanol extraction 3 times, and the method established by 1 h. each time is simple and effective. It is suitable for the extraction of Compound Radix cuspidatum. It can provide reference for the optimization of the extraction process of compound Chinese medicine.
【作者单位】：安徽中医药大学;南京中医药大学附属中西医结合医院;
【基金】：江苏省“六大人才高峰”高层次人才培养对象科研资助项目(2015-YY-011) 江苏省“333高层次人才培养工程”科研资助项目(BRA2015494)
【分类号】：R284;O657.72

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