诺氟沙星在纳米银线基底上的SERS光谱检测

发布时间：2018-05-27 04:26

本文选题：纳米银线 + 诺氟沙星　；参考：《光散射学报》2017年04期

【摘要】：本文以硝酸银为银源,通过多元醇法制备纳米银线,利用扫描电子显微镜、紫外可见分光光度计对纳米银线进行表征,成功制备出直径、长度均一的纳米银线。使用激发波长为785nm的手持式拉曼光谱仪对诺氟沙星溶液进行检测。结果表明该基底具有很好的SERS效果,增强因子达到1.44×107。检测10-3~10-8 mol/L浓度的诺氟沙星溶液时,以浓度和特征峰强度作直线拟合,发现具有较好的线性相关,相关系数R2为0.94101;为进一步考察纳米银线在玻璃片上的分散性,在诺氟沙星(10-3 mol/L)样品的基底上随机取6个点,采集光谱信号。最后,重复检测6个诺氟沙星溶液(10-5 mol/L)样品的拉曼光谱,检验光谱的重现性。实验结果表明,纳米银线在玻璃片上分散性较好,在检测诺氟沙星时体现了较好的重现性。
[Abstract]:In this paper, silver nanowires were prepared by polyol method with silver nitrate as silver source. Nanocrystalline silver wires with uniform diameter and length were successfully prepared by scanning electron microscope and UV-Vis spectrophotometer. Norfloxacin solution was detected by a hand-held Raman spectrometer with excitation wavelength of 785nm. The results show that the substrate has a good SERS effect and the enhancement factor is 1.44 脳 10 ~ 7. In the detection of norfloxacin solution with 10-3 mol/L concentration of 10 ~ (-3) O ~ (-8) mol/L, it was found that there was a good linear correlation between the concentration of norfloxacin and the intensity of the characteristic peak, and the correlation coefficient (R2) was 0.94101. In order to further study the dispersion of silver nanowires on glass, Spectral signals were collected from 6 random points on the substrate of norfloxacin 10-3 mol / L sample. Finally, the Raman spectra of 6 norfloxacin solutions with 10-5 molL / L were determined repeatedly and the reproducibility of the spectra was tested. The experimental results show that the nanocrystalline silver wires have good dispersion on glass and good reproducibility in the detection of norfloxacin.
【作者单位】：广西科技大学生物与化学工程学院;广西科技大学广西糖资源绿色加工重点实验室;广西蔗糖产业协同创新中心;广西科技大学广西高校糖源加工重点实验室;
【基金】：国家自然科学基金(31560466) 广西自然科学基金项目(2016GXNSFAA380108) 广西科技大学硕士研究生创新项目(GKYC201719) 广西高等学校高水平创新团队及卓越学者计划资助(桂教人(2014)7号)
【分类号】：O657.37;TS207.5

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