基于L-半胱氨酸、纳米金自组装的葡萄糖生物传感器的构建

发布时间：2018-05-31 05:56

本文选题：L-半胱氨酸 + 纳米金　；参考：《分析试验室》2017年01期

【摘要】：制备了基于L-半胱氨酸、纳米金自组装的新型葡萄糖生物传感器。用L-半胱氨酸修饰裸金电极,并利用静电吸附的原理固定纳米金,最后通过交联剂使酶固定在修饰后的电极上制成葡萄糖生物传感器。L-半胱氨酸-纳米金以及酶的修饰提高了电极传递电子的能力。通过正交试验选取了最佳的交联条件:6 g/L葡萄糖氧化酶、0.6 g/L过氧化氢酶、0.03%1-乙基-[3-二甲基氨基丙基]碳酰二亚胺盐酸化物、0.03%N-羟基琥珀酰亚胺及p H 7.0的缓冲溶液。该传感器对葡萄糖溶液浓度有良好的线性关系,线性范围为1~16 mmol/L,线性回归方程为I=0.2512c+1.9583,相关系数达到0.9996,具有一定的抗干扰能力。该葡萄糖生物传感器电流大小与葡萄糖浓度呈现良好的线性关系,在临床检验上具有一定的应用前景。
[Abstract]:A novel glucose biosensor based on L-cysteine and nano-gold self-assembly was prepared. The bare gold electrode was modified with L-cysteine and the nanocrystalline gold was immobilized by electrostatic adsorption. Finally, glucose biosensor. L- cysteine-nanocrystalline gold biosensor was made by immobilization of enzyme on the modified electrode by crosslinking agent, and the ability of electron transfer at the electrode was improved by the modification of enzyme. The optimal crosslinking conditions were obtained by orthogonal test. The optimal crosslinking conditions were as follows: 0. 6 g / L glucose oxidase 0.6 g / L catalase 0.03 and 1 ethyl- [3-dimethyl aminopropyl] carbodiimide hydrochlorides 0.03N- hydroxy succinimide and pH 7.0 buffer solution. The linear range of the sensor is 1: 16 mmol / L, the linear regression equation is 0.2512c 1.9583, and the correlation coefficient is 0.9996. The sensor has a certain anti-interference ability. The current of the glucose biosensor has a good linear relationship with glucose concentration and has a certain application prospect in clinical laboratory.
【作者单位】：齐鲁医药学院药学院;山东省高校生物医学工程技术重点实验室;聊城大学化学化工学院;
【分类号】：R446

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