微乳液毛细管电动色谱法测定大鼠滑膜细胞中雷公藤甲素的含量

发布时间：2018-06-01 22:04

本文选题：微乳液毛细管电动色谱 + 滑膜细胞　；参考：《分析化学》2016年01期

【摘要】：建立微乳液毛细管电动色谱法快速测定大鼠滑膜细胞内外液中雷公藤甲素含量的方法。在雷公藤甲素不同给药时间孵育条件下,将滑膜细胞分为空白对照组和给药组,测定滑膜细胞内外液中雷公藤甲素含量,并比较了细胞外液中雷公藤甲素在不同给药时间的含量变化。毛细管电泳运行缓冲液组成:1%(w/V)SDS,3%(V/V)正丁醇,1%(V/V)乙酸乙酯,96%(V/V)5 mmol/L硼砂70 mmol/L磷酸盐溶液,pH 9.0:运行电压:25 kV;压力进样:0.5 psi×6 s;电泳操作温度:25℃;检测波长:214 nm。结果表明,细胞内外液中的雷公藤甲素所呈现的线性关系良好相关系数分别为0.9995和0.9991。当给药24 h时,细胞内外液中的雷公藤甲素浓度分别为0.736和20.745μg/mL。本方法简单准确、灵敏度高、精密度好,可用于滑膜细胞内外液中雷公藤甲素浓度的动态变化规律研究,为进一步研究中药有效成分对滑膜细胞功能和活性的影响提供方法学基础。
[Abstract]:A method for rapid determination of triptolide in rat synovial fluid by microemulsion capillary electrokinetic chromatography was established. Under different administration time of triptolide, synovial cells were divided into blank control group and drug administration group. The content of triptolide in synovial cells was determined. The contents of triptolide in extracellular fluid were compared at different time. The composition of the buffer solution for capillary electrophoresis operation is: 1: 1 / W / V / V / V / V / V / V / V / V / V / V / V / V / V / V / V / V / V / V / V / V / V / V / V / V / V / V / V / V / V / V / V / V / V / V / V / V / V / V / V / V / V / V / V / V / V / V / V / V / V / V / V / V / V / V / V / V / V / V / V / V / V / V / V, The results showed that the linear relationship between triptolide and triptolide was 0.9995 and 0.9991, respectively. At 24 h, the concentration of triptolide was 0.736 渭 g / mL and 20.745 渭 g / mL, respectively. The method is simple, accurate, sensitive and precise. It can be used to study the dynamic change of triptolide concentration in synovial cells. To provide a methodological basis for the further study of the effects of the active components of traditional Chinese medicine on the function and activity of synovial cells.
【作者单位】：福建中医药大学药学院;
【基金】：国家自然科学基金项目(No.81202913) 福建省科技厅社会发展重点项目(No.2013Y0057) 福建省卫生系统中青年骨干人才培养项目(No.2014-ZQN-JC-27) 福建中医药大学校管科研课题-重点学科专项(No.X2014133-学科)资助~~
【分类号】：R285.5;O657.7

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