高灵敏检测葡萄糖的新型荧光纳米传感器

发布时间：2018-06-05 23:34

本文选题：有机荧光探针 + 荧光传感器　；参考：《分析化学》2017年06期

【摘要】：构建了一种用于高灵敏检测葡萄糖的新型荧光纳米传感器。在辣根过氧化物酶(HRP)的催化下,H2O2氧化3,3',5,5'-四甲基联苯胺(TMB),生成具有强吸光性质的TMB多聚体,导致1-氧-1H-非那烯-2,3-二腈(1-Oxo-1H-phenalene-2,3-dicarbonitrile,OPD)分子的荧光发生淬灭,基于此实现H_2O_2的定量检测,线性范围分别为0.05~0.80μmol/L和1~10μmol/L,检出限(3")为0.02μmol/L。由于葡萄糖氧化酶(Gox)可催化葡萄糖分解产生H_2O_2,基于此可以实现葡萄糖分子的定量检测,线性范围分别为0.1~3.0μmol/L和4.0~30μmol/L,检出限(3")为0.02μmol/L。将本方法用于实际血清样品中葡萄糖的定量检测,结果与临床检测结果相符。
[Abstract]:A novel fluorescent nanosensor for the sensitive detection of glucose was constructed. Under the catalysis of horseradish peroxidase (horseradish peroxidase), H _ 2O _ 2 was oxidized by H _ 2O _ 2, and the TMB polymer with strong absorptivity was formed, which resulted in the fluorescence quenching of 1-oxo-1H-phenalene-23-dicarbonitrile OPDs, based on which the quantitative detection of H_2O_2 was realized. The linear range is 0. 05 渭 mol / L for 0. 80 渭 mol/L and 1 for 10 渭 mol / L, respectively. The detection limit is 0. 02 渭 mol / L. Because glucose oxidase (Gox) can catalyze the decomposition of glucose to produce H _ 2O _ 2, the quantitative detection of glucose molecules can be realized based on this. The linear ranges are 0.1 渭 mol/L and 4.0 渭 mol / L, respectively, and the detection limit is 3 ") of 0.02 渭 mol 路L ~ (-1). The method has been applied to the quantitative determination of glucose in real serum samples, and the results are in agreement with the clinical results.
【作者单位】：陆军总医院消化内科;北京化工大学理学院化工资源有效利用国家重点实验室;
【基金】：北京化工大学中央高校基本科研业务费项目(Nos.buctrc201507,buctrc201608) 国家自然科学基金项目(No.21505003)资助
【分类号】：O657.3;TP212

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