三种提取藏红花废弃物挥发油方法比较

发布时间：2018-06-06 13:40

本文选题：藏红花 + 花瓣　；参考：《四川大学学报(自然科学版)》2017年06期

【摘要】：采用水蒸汽蒸馏法、超声辅助浸提法和索氏提取法三种方式提取藏红花花瓣及雄蕊中的挥发油,用GC-MS分离鉴定成分并测定各成分的相对含量.结果显示共鉴定出化合物49种,水蒸汽蒸馏法、超声辅助浸提法和索氏提取法分别分离鉴定出43、27、21种组分,且共有6种相同成分.挥发油的主要成分为棕榈酸、亚油酸、亚麻酸.水蒸汽蒸馏法提取组分中含有如芳樟醇、优葛缕酮、柏木脑等独有成分,且藏红花醛含量可达40.31%,索氏提取法挥发油提取率最高,为6.04%.DPPH自由基清除实验表明,水蒸汽提取挥发油在280μg/mL时清除率效果表现最佳为44.17%.还原力测定实验及总抗氧化能力实验中,索氏提取物表现出的抗氧化能力最强.
[Abstract]:The volatile oil from the petals and stamens of saffron was extracted by steam distillation, ultrasonic assisted extraction and Soxhlet extraction. The components were isolated and identified by GC-MS and the relative contents of each component were determined. The results showed that 49 compounds were identified, water vapor distillation method, ultrasonic assisted extraction method and Soxhlet extraction method were used to separate and identify 41 compounds, and there were 6 same components. The main components of volatile oil are palmitic acid, linoleic acid and linolenic acid. The components extracted by steam distillation method contain unique components such as linalool, ursolone, cypress and so on, and the content of saffron aldehyde can reach 40.31. The extraction rate of essential oil by Soxhlet extraction method is the highest, which is indicated by the scavenging experiment of 6.04%.DPPH free radical. The removal rate of volatile oil extracted by steam at 280 渭 g/mL was 44.17. Soxhlet extract showed the strongest antioxidant ability in reducing capacity test and total antioxidant ability test.
【作者单位】：四川大学生命科学学院生物资源与生态环境教育部重点实验室;宜宾学院香料植物资源开发与利用四川省高校重点实验室;
【基金】：香料植物资源开发与利用四川省高校重点实验室开放基金资助项目(2016XLZ002) 西红花规模化生产技术服务(HS20100601106684)
【分类号】：O657.63;TQ914.3

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