碳点与异硫氰酸荧光素相互作用、选择性细胞成像及抗病毒研究

发布时间：2018-06-07 21:51

  本文选题：碳点 + 异硫氰酸荧光素　； 参考：《华中农业大学》2017年硕士论文

【摘要】：碳点(CDs)作为一种新型的纳米荧光材料,具备优异的光学性质和良好的生物相容性,在生化检测、生物成像、活体诊断及治疗等领域引起研究者的广泛关注。CDs在比率型荧光探针及抗病毒等领域的研究仍具有很大的潜力。鉴于此,本文在合成CDs的基础上,研究了CDs与异硫氰酸荧光素(FITC)的相互作用,并探讨了CDs在细胞成像及抗病毒等方面的应用,具体研究内容如下:1.利用光谱技术研究了CDs和FITC在pH为5.1、7.0和9.0条件下的相互作用。当CDs和不同浓度的FITC进行直接混合后,会导致CDs的荧光强度逐渐地被猝灭,而CDs的荧光寿命保持不变,通过对紫外-可见吸收光谱及荧光寿命的分析,证实了CDs和FITC之间pH依赖的内滤效应(IFE)。然而,CDs和FITC进行化学偶联后,导致了CDs荧光寿命的下降,表明了CDs和FITC之间发生了荧光共振能量转移(FRET)。该研究不仅揭示了不同pH条件下CDs和FITC之间不同的作用形式,同时也为进一步研究纳米粒子和荧光团之间的相互作用提供了理论指导。2.以温和的方式合成了两种不同的CDs,在未经进一步修饰的情况下可以选择性地进入细胞质和整个细胞。通过在CDs的水热合成过程中加入适量的脲,不仅使得CDs的表面电位由负电位变为电中性,同时制备得到的CDs显示出不同的荧光。细胞成像应用中,蓝色荧光的CDs(b-CDs)只能进入细胞的细胞质,而青色荧光的CDs(c-CDs)可以分布到整个细胞,包括细胞核。此外,我们还研究了两种CDs对伪狂犬病毒(PRV)的抗病毒活性,结果显示b-CDs具有更好的抗病毒活性。该研究以一种温和新颖的方式制备得到了用于细胞选择性成像的两种CDs,研究结果有望进一步促进CDs在纳米医药领域的应用。
[Abstract]:As a new type of nano-fluorescent material, carbon Dot (CDS) has excellent optical properties and good biocompatibility, in biochemical detection, biological imaging, In vivo diagnosis and treatment have attracted wide attention of researchers. CDs still have great potential in the research of ratio fluorescent probe and antivirus. Based on the synthesis of CDs, the interaction between CDs and fluorescein isothiocyanate (FITC) was studied, and the applications of CDs in cell imaging and antivirus were discussed. The interaction between CDs and FITC at pH 5.1 and 9.0 was studied by spectroscopic technique. When CDs are mixed directly with FITC at different concentrations, the fluorescence intensity of CDs is gradually quenched, while the fluorescence lifetime of CDs remains unchanged. The internal filtration effect of pH dependence between CDs and FITC was confirmed. However, chemical coupling of CDs with FITC leads to a decrease in fluorescence lifetime of CDs, which indicates that fluorescence resonance energy transfer occurs between CDs and FITC. This study not only reveals the different forms of interaction between CDs and FITC at different pH, but also provides theoretical guidance for the further study of the interaction between nanoparticles and fluorescent groups. Two different CDss were synthesized in a mild manner, which can selectively enter the cytoplasm and the whole cell without further modification. By adding a proper amount of urea to the hydrothermal synthesis of CDs, not only the surface potential of CDs is changed from negative potential to electric neutral, but also the CDs show different fluorescence. In cell imaging applications, the blue fluorescent CDslb-CDS can only enter the cytoplasm of the cell, while the cyan fluorescence CDstc-CDS can be distributed throughout the cell, including the nucleus. In addition, we also studied the antiviral activity of two CDs against pseudorabies virus (PRV). The results showed that b-CDs had better antiviral activity. Two CDss for cell selective imaging were prepared in a mild and novel way. The results of this study are expected to further promote the application of CDs in the field of nanopharmaceuticals.
【学位授予单位】：华中农业大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：O657.3

【参考文献】

相关硕士学位论文 前1条

1 王莉;荧光碳量子点的制备及其发光机理的研究[D];中国计量学院;2015年

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本文编号：1992901