透明质酸的功能改性及其应用研究

发布时间：2018-06-11 15:23

本文选题：透明质酸 + 双亲大分子　；参考：《江南大学》2017年硕士论文

【摘要】：大分子自组装是制备功能性纳米颗粒的一种有效手段,2005年Armes团队首次以大分子自组装制备聚合物胶体粒子作为颗粒乳化剂,用于稳定油/水乳状液,并逐渐成为研究热点。有别于聚合物颗粒乳化剂,生物基颗粒乳化剂因其可降解、环境友好等特性迅速进入人们的视线。透明质酸(HA)作为天然大分子之一,具有良好生物相容性、易降解性、卓越保湿性等优势,在许多领域有不可估量的应用前景。但HA自身亲水性强、功能性单一的缺点限制了它的应用范围,因此通常用物理或化学方法对其进行改性,以改变HA的物化性质赋予其更多功能。本论文利用光敏单体疏水改性HA,研究改性HA在选择性溶剂中的自组装行为,并且以改性HA胶体粒子为颗粒乳化剂,探究其在油水界面的二次组装行为;最后,选取合适的改性HA胶体粒子用于化妆品膏霜实现了HA颗粒乳化剂的实际应用。本论文主要由以下三个部分组成:1、肉桂酸改性透明质酸自组装胶体粒子制备及其乳化性能研究本章利用光敏单体肉桂酸(CA)疏水改性HA,制得双亲性的HA衍生物HA-CA,在选择性溶剂(DMSO/H2O)中可自组装得到HA-CA胶体粒子。通过红外光谱、核磁共振氢谱和紫外谱图确定HA-CA的化学结构和性质;通过紫外分光光度计追踪HA-CA的组装过程,利用纳米粒度及Zeta电位分析仪、透射电镜(TEM)、视频光学接触角测量仪对HA-CA胶体粒子的结构、形貌、亲疏水性等进行了表征;以HA-CA胶体粒子为颗粒乳化剂研究其在油水界面的二次组装行为,考察了水相p H、盐浓度、油相类型等因素对胶体粒子在油水界面组装行为的影响,并通过苯乙烯油相固化法表征了胶体粒子在油水界面的形貌;通过细胞实验研究胶体粒子以及所制备乳液的细胞相容性。结果显示:HA-CA在UVB波段具有紫外吸收;HA-CA胶体粒子作为颗粒乳化剂可制备O/W型Pickering乳液,且胶体粒子具有一定的保湿性和良好的细胞相容性。2、香豆素衍生物改性透明质酸自组装胶体粒子制备及其乳化性能研究本章利用香豆素衍生物7-(2-羟基乙氧基)-4-甲基香豆素(HEMC)作为光敏单体疏水改性HA获得HA衍生物HA-HEMC,双亲性HA-HEMC在选择性溶剂(DMSO/H2O)中可自组装形成HA-HEMC胶体粒子。通过红外光谱、核磁共振氢谱以及紫外谱图对HA-HEMC的化学结构和性质进行表征;通过紫外分光光度计研究HA-HEMC的组装行为,利用动态激光光散射(DLS)、纳米粒度及Zeta电位分析仪、透射电镜(TEM)等对HA-HEMC胶体粒子的结构、形貌和性质进行了表征;将HA-HEMC胶体粒子作为颗粒乳化剂稳定油水界面,考察了胶体粒子取代度(DS)、质量浓度、油相类型、水相pH、盐浓度等对胶体粒子稳定油水界面的影响,并通过GTT法表征胶体粒子在油水界面的形貌。结果显示:HA-HEMC在UVA和UVB波段均具有紫外吸收;该胶体粒子能够在较宽pH范围内(3~11)稳定O/W型Pickering乳液,且所得乳液有良好的耐盐性;此外,HA-HEMC胶体粒子还能够稳定多种油-水体系。3、HA-HEMC自组装胶体粒子作为颗粒乳化剂制备防晒膏霜的应用研究本章选取HA-HEMC胶体粒子作为研究对象,通过紫外分光光度计检测胶体粒子的光敏性;通过称重法检测胶体粒子的保湿性;通过细胞实验研究胶体粒子的生物相容性。将HA-HEMC胶体粒子加入化妆品膏霜中,通过感官评价、pH值测定、稳定性测试等考察了化妆品膏霜的品质;通过紫外透过率测试评价化妆品膏霜的防晒性能。结果显示:HA-HEMC胶体粒子具有光敏性、保湿性以及细胞相容性,是一种新型的防晒化妆品原料,且随着胶体粒子加入量的增加膏霜的品质及防晒性能都得到提升。
[Abstract]:Macromolecule self-assembly is an effective means to prepare functional nanoparticles. In 2005, Armes team prepared polymer colloid particles as particle emulsifier for the first time, used to stabilize oil / water emulsion, and gradually became a hot spot. As one of the large natural molecules, hyaluronic acid (HA), as one of the large natural molecules, has the advantages of good biocompatibility, easy degradation and excellent moisture retention, and has an inestimable application prospect in many fields. However, HA has a strong hydrophilic property and a single functional disadvantage, so it is usually used in physics. Modify the physical and chemical properties of HA by chemical method to change its physical and chemical properties. In this paper, the self assembly behavior of modified HA in the selective solvent was studied by using the hydrophobic modified HA, and the modified HA colloid particles were used as particle emulsifier to explore the two assembly behavior of the modified HA particles in the oil and water surface. Finally, the appropriate modification was selected. The application of HA colloid particles to cosmetic cream has realized the practical application of HA particle emulsifier. This paper is mainly composed of three parts: 1, preparation and emulsification of self assembled colloidal particles of cinnamic acid modified hyaluronic acid in this chapter, a amphiphilic HA derivative HA-CA is prepared by using photosensitive monomer cinnamic acid (CA) hydrophobic modified HA. HA-CA colloid particles can be self assembled by self assembly in the sex solvent (DMSO/H2O). The chemical structure and properties of HA-CA are determined by IR, NMR and UV spectra. The UV spectrophotometer is used to track the assembly process of HA-CA, using the nanoscale and Zeta potential analyzer, transmission electron microscopy (TEM), and the video optical contact angle measuring instrument to HA-CA The structure, morphology, hydrophobicity of the colloid particles were characterized, and the two assembly behavior of HA-CA colloid particles as particle emulsifier on the oil and water interface was studied. The effects of the water phase P H, salt concentration and oil phase type on the assembly behavior of colloidal particles on the oil and water interface were investigated, and the colloidal particles were characterized by the styrene oil phase curing method. The morphology of the seed in the oil and water interface; the cell compatibility of colloid particles and the prepared emulsion by cell experiment. The results show that HA-CA has UV absorption in the UVB band; HA-CA colloid particles can be used as particle emulsifier to prepare O/W Pickering emulsion, and the colloid particles have a definite moisture retention and good cellular compatibility.2, sweet bean The preparation and emulsification properties of self assembled colloidal particles of the modified hyaluronic acid derivatives were studied in this chapter, using the coumarin derivative 7- (2- hydroxy ethoxy) -4- methyl coumarin (HEMC) as the hydrophobic modified HA of the photosensitive monomer to obtain the HA derivative HA-HEMC, and the amphiphilic HA-HEMC can be self-assembled to form HA-HEMC colloid particles in the selective solution (DMSO/H2O). The chemical structure and properties of HA-HEMC were characterized by IR, NMR and UV spectra, and the assembly behavior of HA-HEMC was studied by UV spectrophotometer. The structure, morphology and properties of HA-HEMC colloid particles were studied by dynamic laser light scattering (DLS), nanoscale and Zeta potential analyzer, transmission electron microscope (TEM) and so on. The effects of colloid particle substitution (DS), mass concentration, oil phase type, water phase pH and salt concentration on the stability of oil and water interface were investigated by using colloidal particles as particle emulsifier to stabilize oil water interface, and the morphology of colloid particles in oil and water interface was characterized by GTT method. The results showed that HA-HEMC was in both UVA and UVB bands. The results showed that HA-HEMC was both in UVA and UVB bands. UV absorption, the colloid particles can stabilize O/W Pickering emulsion in a wide pH range (3~11), and the emulsion has good salt tolerance. In addition, HA-HEMC colloid particles can also stabilize a variety of oil water system.3, HA-HEMC self assembled colloid particles as particle emulsifier to prepare sunscreen cream for the application of HA-HEMC colloid As the object of study, the photosensitivity of colloid particles was detected by ultraviolet spectrophotometer. The moisture retention of colloid particles was detected by weighing method, and the biocompatibility of colloid particles was studied by cell experiment. HA-HEMC colloid particles were added to cosmetics cream, and cosmetics were examined by sensory evaluation, pH value determination, stability test and so on. The quality of the cream is evaluated by the UV transmittance test. The results show that the HA-HEMC colloid particles are photosensitive, moisturizing and cellular compatibility. It is a new type of raw material for sunscreen cosmetics, and as the addition of colloid particles increases the quality of cream and the performance of sunscreen are improved.
【学位授予单位】：江南大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：O629.9;TQ658.24

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