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几种氮、氧杂环类染料的设计合成及其在检测方面的应用

发布时间:2018-06-12 05:59

  本文选题:比色/荧光探针 + 静电纺丝 ; 参考:《郑州大学》2016年硕士论文


【摘要】:近年来,功能化荧光染料在材料科学和生命科学中有着广泛的应用。其中,比色/荧光分子探针因其灵敏度高、选择性好、无损伤检测、操作方便等优点而成为非常有用的检测分析技术。然而,在实际应用中分子探针的功能会受到诸如响应速度慢、背景干扰大、抗干扰能力差等问题的限制。本论文针对目前荧光探针存在的不足,设计合成了三种新型的分子探针,具体如下:1、设计合成出一种锌离子单核配合物并且可以作为高选择性裸眼识别焦磷酸根(PPi)的化学传感器。基于酮式-烯醇式转换过程,PPi的加入使传感器的颜色从红色变为无色。此外,将由络合物制备的静电纳米纤维浸入到焦磷酸根溶液之后,纤维膜的颜色变化迅速,可实现原位即时检测PPi。纤维膜的颜色变化迅速是由于纳米纤维膜比表面积较大引起的。2、基于1,8-萘酰亚胺设计合成了一种高选择性高灵敏度的半胱氨酸(Cys)、同型半胱氨酸(Hcy)、谷胱甘肽(GSH)及苯硫酚荧光分子探针。该探针能够在0-6.0μM范围内对半胱氨酸(Cys)定量检测,并且检出限低至1.6 nM。实验证实该探针能够在试纸上检测Cys以及在细胞中选择性识别Cys、Hcy、GSH。同时该探针能快速的识别苯硫酚,表现出了很好的选择性及抗干扰能力(分析物包括亲核试剂、脂肪族硫醇、阴离子及金属离子)。对苯硫酚的定量检测的范围为0-4.0μM,检出限为10.3 nM。该探针能够在试纸上及在细胞中选择性识别苯硫酚。并且,根据探针分子与半胱氨酸(Hcy)、同型半胱氨酸(Hcy)、谷胱甘肽(GSH)和苯硫酚的响应速率不同,可以成功的将苯硫酚与Cys、Hcy、GSH区分开来。3、以9,10-苯醌为起始原料设计合成出一种新颖的水合肼比率/比色/荧光探针。随着水合肼的加入,该探针分子的颜色和荧光均发生变化,可以作为一种双通道的荧光探针,能够降低检测环境,仪器等多方面的影响。在细胞中,随着水合肼的加入,该探针的荧光也发生比率的变化。此外,该探针低毒,表现出很好的生物相容性。
[Abstract]:In recent years, functionalized fluorescent dyes have been widely used in material science and life science. Among them, colorimetric / fluorescent probe has become a very useful detection and analysis technology because of its high sensitivity, good selectivity, no damage detection, easy operation and so on. However, in practical applications, the function of molecular probes is limited by such problems as slow response speed, large background interference and poor anti-jamming ability. In this paper, three novel molecular probes have been designed and synthesized in allusion to the shortcomings of fluorescent probes. As follows: 1, a zinc mononuclear complex has been designed and synthesized and can be used as a highly selective chemical sensor for optically recognized pyrophosphate PPis. Ketone-enol conversion process based on the addition of PPi sensor color from red to colorless. In addition, when the electrostatic nanofibers prepared by the complex were immersed in the pyrophosphate solution, the color of the fiber membrane changed rapidly, and the in situ detection of PPis could be realized. The rapid color change of fiber membrane is caused by the large surface area of nanofiber membrane. Based on the design and synthesis of high selectivity and high sensitivity cysteine cystine, homocysteine cystine, glutathione GSH and benzene based on the design of 1 / 8- naphthalene imide, a kind of high selectivity and high sensitivity cysteine, homocysteine, glutathione (GSH) and benzene were synthesized. Thiophenol fluorescence probe. The probe can be used to detect cysteine in the range of 0-6.0 渭 M, and the detection limit is as low as 1.6 nm. The results showed that the probe could detect Cys on the test paper and selectively recognize cystatin GSH in the cells. At the same time, the probe can recognize phenylthiophenol quickly, showing good selectivity and anti-interference ability. The analytes include nucleophilic reagent, aliphatic mercaptan, anion and metal ion. The range of quantitative detection of p-phenylthiophenol was 0-4.0 渭 M and the detection limit was 10.3 nm. The probe can selectively recognize phenylthiophenol on test paper and in cells. In addition, according to the response rates of probe molecules to cysteine, homocysteine, glutathione, and phenylthiophenol, the response rates of the probe molecules were different from those of cysteine, homocysteine, glutathione and phenylthiophenol. A novel hydrazine hydrate / colorimetric / fluorescent probe was designed and synthesized by separating phenylthiophenol from cystoxy GSH. 3. A new type of hydrazine hydrate / colorimetric / fluorescent probe was designed and synthesized from 9 ~ (10) -benzoquinone. With the addition of hydrazine hydrate, the color and fluorescence of the probe molecule change, which can be used as a two-channel fluorescence probe, which can reduce the detection environment, instruments and other aspects of the impact. The fluorescence of the probe also changed with the addition of hydrazine hydrate. In addition, the probe is low toxic and shows good biocompatibility.
【学位授予单位】:郑州大学
【学位级别】:硕士
【学位授予年份】:2016
【分类号】:O657.3

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