HPLC法测定利心丸中毛蕊花糖苷的含量
发布时间:2018-06-12 11:46
本文选题:利心丸 + 毛蕊花糖苷 ; 参考:《中国药房》2016年33期
【摘要】:目的:建立测定利心丸中毛蕊花糖苷含量的方法。方法:采用高效液相色谱法。色谱柱为ZORBAX SB-Aq,流动相为乙腈-0.1%磷酸溶液(16∶84,V/V),流速为1.0 ml/min,检测波长为334 nm,柱温为30℃,进样量为10μl。结果:毛蕊花糖苷检测进样量线性范围为0.254 4~1.526 4μg(r=0.999 7);定量限为1.358 6μg/ml,检测限为0.407 6μg/ml;精密度、稳定性、重复性试验的RSD2%;加样回收率为95.06%~97.31%(RSD=1.05%,n=6)。结论:该方法操作简便、结果准确,适用于测定利心丸中毛蕊花糖苷的含量。
[Abstract]:Objective: to establish a method for the determination of Rutherin in Lixin pills. Methods: high performance liquid chromatography was used. The chromatographic column was ZORBAX SB-Aq.The mobile phase was acetonitrile-0.1% phosphoric acid solution (16: 84 V / V), the flow rate was 1.0 ml / min, the detection wavelength was 334 nm, the column temperature was 30 鈩,
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