新型稀土配合物荧光探针的设计合成与应用

发布时间：2018-06-14 14:50

本文选题：稀土配合物 + 荧光探针　；参考：《大连理工大学》2016年硕士论文

【摘要】：近20年间,以稀土荧光配合物作为探针的时间分辨荧光测定技术取得了长足的发展,并且在生命科学研究和医学临床诊断中获得了越来越多的关注。同时,单线态氧(lO2)作为一种重要的活性氧在许多光化学和光生物化学反应过程中都扮演着重要的角色,特别是在癌症的光动力学治疗技术(PDT)中单线态氧起着十分重要的作用。本学位论文中,.将9,10-二甲基蒽作为单线态氧识别基团,与稀土荧光β二酮铕配合物共价偶联,设计合成了一种新型的β-二酮铕作为配位体检测单线态氧的时间分辨荧光探针,[Eu(pfdap)3(tpy)] (Hpfdap:1,1,1,2,2-五氟-5-(9',10'-二甲基-2'-乙酰基)-3,5-乙酰丙酮；tpy：2,2',2”-三联吡啶),建立了基于该探针的单线态氧时间分辨荧光测定及其在活细胞内检测单线态氧的荧光生物成像技术。由于探针结构中9,10-二甲基蒽基团对铕配合物荧光的淬灭作用,该探针几乎没有荧光,而与单线态氧特异性响应之后,9,10-二甲基蒽基团被氧化,荧光淬灭作用消失,铕配合物长寿命的荧光得以恢复,其荧光强度增加68倍。该探针选择性好,灵敏度和信噪比高,适于复杂生物体系中单线态氧的测定。利用时间分辨荧光显微镜成像技术,该探针成功用于肝癌细胞HepG2细胞在癌症光动力学治疗过程中,光敏化剂5-氨基酮戊酸诱导产生的单线态氧实时原位的示踪检测。通过共聚焦显微镜和与商业化线粒体靶向荧光染料共染色实验,进一步证明了[Eu(pfdap)3(tpy)]探针的线粒体靶向定位功能,为细胞器靶向稀土配合物荧光探针的开发提供了新的思路。
[Abstract]:In recent 20 years, time-resolved fluorimetry with rare earth fluorescent complexes as a probe has made great progress, and has gained more and more attention in life science research and medical clinical diagnosis. At the same time, as a kind of important reactive oxygen species, singlet oxygen species play an important role in many photochemical and photochemical reactions, especially in PDT (photodynamic therapy of cancer). In this thesis. The single line oxygen recognition group of 9 ~ (10) -dimethyl anthracene was covalently coupled with a rare earth fluorescent 尾 -diketone europium complex. A new type of 尾 -dione europium has been designed and synthesized as a time-resolved fluorescence probe for the detection of singlet oxygen. A novel time-resolved fluorescence technique for the detection of singlet oxygen in living cells and a fluorescence biometric imaging technique for the detection of singlet oxygen in living cells have been developed based on tpy: 2 / 2 "- tripyridine. Because the fluorescence of europium complex was quenched by the 9 ~ (10) -dimethyl anthracene group in the structure of the probe, the probe had almost no fluorescence, but the fluorescence quenching effect disappeared after the specific response to the singlet oxygen, and the fluorescence quenching effect disappeared after the oxidation of the 9 ~ (10) -dimethyl anthracene group. The long lifetime fluorescence of europium complex can be recovered and its fluorescence intensity is increased by 68 times. The probe has good selectivity, high sensitivity and signal-to-noise ratio, and is suitable for the determination of singlet oxygen in complex biological systems. Using time-resolved fluorescence microscopy, the probe was successfully used to detect in situ single linear oxygen produced by 5-aminolevulinic acid (5-aminolevulinic acid) during photodynamic therapy of HepG2 cells. By means of confocal microscopy and co-staining with commercial mitochondria targeted fluorescent dyes, the mitochondrial targeting localization function of Eupfdapia 3tpy3 probe is further proved, which provides a new idea for the development of organelle targeting rare earth complex fluorescent probes.
【学位授予单位】：大连理工大学
【学位级别】：硕士
【学位授予年份】：2016
【分类号】：O641.4;O657.3

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