分子印迹凝胶光子晶体的制备及其性能研究

发布时间：2018-06-14 15:49

  本文选题：光子晶体 + “三明治”法　； 参考：《江南大学》2016年硕士论文

【摘要】：分子印迹是一种构建具有特异性识别位点的聚合物的有效方法。由于聚合物中形成的纳米孔穴在尺寸、空间形状以及作用点上均与印迹分子相匹配,因此可选择性识别被印迹分子。反蛋白石光子晶体由于具有三维有序孔结构,与可见光发生强烈相互作用产生Bragg衍射,呈现出鲜艳的结构颜色。分子印迹凝胶光子晶体(MIPHs)是将反蛋白石光子晶体的三维有序结构与分子印迹聚合物水凝胶相结合发展起来的。MIPHs的体积随着对环境中目标分子的特异性识别吸附发生变化,引起光子晶体的晶格参数发生明显改变,造成光子晶体的Bragg衍射峰偏移,同时产生肉眼即可观察的颜色变化从而实现对待测物的半定量分析。本文制备了两类重要分子L-色氨酸(L-Trp)、叶酸(FA)印迹的凝胶光子晶体,并对其响应性能进行了测试、表征,表明制得的MIPHs具有高选择性、高灵敏性、信号自表达、快速响应以及可再生的优点。具体研究内容与结果如下:(1)采用经典的胶体晶体模板法制备MIPHs,借助两种前驱液填充方法“三明治”法和“小角度倾斜渗入”法,均可获得良好的反蛋白石结构。“三明治”法由于操作更为简单方便,为此本文研究的MIPHs采用“三明治”法制得。此外,制备出的MIPHs可与基底剥离,改善了其溶胀-收缩性,并且方便使用。(2)以丙烯酰胺(AM)为功能单体,光引发聚合制备了L-色氨酸(L-Trp)分子印迹凝胶光子晶体(L-MIPHs),考察了MIPHs对印迹分子的选择性。随着目标分子L-Trp浓度增大,分子印迹凝胶光子晶体的Bragg衍射峰红移83 nm,并能肉眼观察到其在不同浓度的目标分子溶液中呈现不同的颜色。(3)以丙烯酰胺(AM)、马来酸酐改性环糊精(MAH-β-CD)为功能单体,光引发聚合制备了基于β-CD的L-色氨酸(L-Trp)分子印迹凝胶光子晶体(CD-MIPHs)。采用响应面法,利用中心组合实验设计,基于有限数目的实验评估了影响MIPHs性能的主要影响因素,并预测了最优聚合物组成。通过实验发现随着待测液中目标分子L-Trp浓度不断增大,MIPHs的Bragg衍射峰红移126 nm,并对两种构型的色氨酸表现出良好的手性识别性能。(4)制备出叶酸(FA)与马来酸酐改性环糊精(MAH-β-CD)的FA-MAH-β-CD包合物,以丙烯酰胺(AM)、FA-MAH-β-CD包合物为功能单体,光引发聚合制备了基于β-CD的叶酸(FA)分子印迹凝胶光子晶体(F-MIPHs)。制备出的叶酸(FA)分子印迹凝胶光子晶体最低检测限可达到10-12 mol/L。
[Abstract]:Molecular imprinting is an effective method for the construction of polymers with specific identification sites. Because the nano holes formed in the polymer are matched with the imprinted molecules in size, space shape and the point of action, the molecularly imprinted molecules can be identified. The inverse opal photonic crystal has a three-dimensional ordered pore structure and visible light. The strong interaction produces Bragg diffraction, showing a bright structure color. Molecular imprinted gel photonic crystal (MIPHs) is the volume of.MIPHs which combines the three-dimensional ordered structure of the inverse opal photonic crystal and the molecularly imprinted polymer hydrogel, and changes with the specific recognition and adsorption of the target molecules in the environment. The lattice parameters of the photonic crystal change obviously, causing the Bragg diffraction peak shift of the photonic crystal, and producing the color changes that can be observed by the naked eye so as to realize the semi quantitative analysis of the measured objects. This paper prepared two kinds of important molecular L- tryptophan (L-Trp), folic acid (FA) imprinted gel photonic crystal and its response performance. The results are as follows: (1) the preparation of MIPHs by the classical colloidal crystal template method and the "sandwich" method and the "small angle inclined infiltration" method with the help of two kinds of precursor filling methods. The results are as follows: (1) 1) the preparation of the colloidal crystal template method by the classical colloidal crystal template method, and the "sandwich" method and the "small angle inclined infiltration" method by means of the method of filling the precursor. A good inverse opal structure can be obtained. "Sandwich" method is more simple and convenient to operate, for this purpose, the MIPHs method used in this paper is obtained by "sandwich" method. In addition, the prepared MIPHs can be stripped from the substrate, improve its swelling and shrinkage, and be convenient to use. (2) L- is used as a functional monomer and photopolymerization is used to prepare L-. The molecular imprinted gel photonic crystal (L-MIPHs) of tryptophan (L-Trp) was used to investigate the selectivity of MIPHs to the imprinted molecules. As the L-Trp concentration of the target molecule increased, the Bragg diffraction peak of the Molecularly Imprinted Photonic Crystal was red shift 83 nm, and it could be observed by the naked eye to show different colors in the solution of different concentrations of the target molecules. (3) AM A L- tryptophan (L-Trp) Molecularly Imprinted Photonic Crystal (CD-MIPHs) based on L- tryptophan (L-Trp) was prepared by photopolymerization of maleic anhydride modified cyclodextrin (MAH- beta) as a functional monomer. The response surface method was used to evaluate the main influence factors on the performance of MIPHs based on the experimental design of the central combination, and the optimal polymerization was evaluated and the optimal polymerization was predicted. It is found that the Bragg diffraction peak of MIPHs has a red shift of 126 nm with the increasing concentration of the target molecule L-Trp in the solution, and a good chiral recognition performance is shown for the two types of tryptophan. (4) the preparation of the FA-MAH- beta -CD inclusion complex of folic acid (FA) and maleic anhydride modified cyclodextrin (MAH- beta -CD), with acrylamide (AM), FA-M AH- beta -CD inclusion complex is a functional monomer and photopolymerization based on beta -CD (FA) molecular imprinted gel photonic crystal (F-MIPHs) is prepared. The minimum detection limit of the prepared folic acid (FA) molecularly imprinted gel photonic crystal can reach 10-12 mol/L.
【学位授予单位】：江南大学
【学位级别】：硕士
【学位授予年份】：2016
【分类号】：O631.3;O734
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本文编号：2018011