赭曲霉毒素A电化学传感检测新方法建立

发布时间：2018-06-15 17:52

本文选题：赭曲霉毒素A + 电化学核酸传感器　；参考：《合肥工业大学》2017年硕士论文

【摘要】：赭曲霉毒素A是一种由青霉属和曲霉属所产生的有毒代谢产物,广泛存在于谷物等农产品中,能侵害人体肾脏和肝脏,并引发大多数哺乳动物产生癌症、肾中毒、肝脏中毒以及免疫毒性危害等。因此,建立快速、灵敏、便捷的赭曲霉毒素A检测方法对保护人类健康具有重要的作用。本研究选取赭曲霉毒素A(OTA)为研究对象,一方面通过利用核酸适配体以达到快速检测目的;另一方面通过选取合适的样品预处理以及合适的纳米材料来实现检测范围的调节以及信号的扩增。其具体研究内容如下:(1)基于辅助核酸适配体电化学传感器对赭曲霉毒素A的超宽范围可调控检测利用辅助核酸适配体以及固定电极表面的核酸适配体对赭曲霉毒素A的特异性识别实现超宽范围可控型检测。该电化学核酸传感器的检测范围是10-8至100ppb,共有11个数量级的宽度。并且,所有浓度的检测信号响应都在同一个电流区间内,实验操作简单,无需信号放大操作即可实现宽范围的检测。在最优实验条件下,对实际样品检测同样适用。该设计利用核酸适配体的识别,同样适用于其他有核酸适配体的目标物的检测。(2)基于双重金增强的电化学核酸传感器检测赭曲霉毒素A利用金纳米粒子表面偶联核酸探针对电极表面进行不同的自组装实现OTA的快速、灵敏检测。通过利用金纳米粒子的高比表面积作用富集更多的信号分子实现检测信号的增强;再通过电极表面组装表面偶联核酸探针的金纳米粒子使得核酸适配体的自组装量得到提高,从而实现检测宽度的提高。该电化学核酸适配体的检测范围在0.001至1000 ppb,与基础型相比有2个数量级的拓宽。且对实际样品的检测可以适用,通过改变核酸适配体序列即可应用与其他目标物的检测。
[Abstract]:Ochratoxin A is a toxic metabolite produced by Penicillium and Aspergillus. It is widely found in agricultural products such as cereals. It can damage human kidneys and liver, and cause cancer and nephrotoxicity in most mammals. Liver poisoning and immune toxicity. Therefore, to establish a rapid, sensitive and convenient method for detection of ochratoxin A plays an important role in the protection of human health. In this study, ochratoxin OTAA was selected as the research object, on the one hand, the aptamer of nucleic acid was used to achieve the purpose of rapid detection. On the other hand, we can adjust the detection range and amplify the signal by selecting the appropriate sample pretreatment and the appropriate nanomaterials. The specific contents of this study are as follows: (1) based on the electrochemical sensor of auxiliary aptamer of nucleic acid for the detection of ochratoxin A in a very wide range, the auxiliary aptamer of nucleic acid and the aptamer of nucleic acid on the surface of fixed electrode can be used for the detection of ochratoxin on the surface of the electrode. The specific recognition of hormone A can achieve a very wide range of controllable detection. The electrochemical nucleic acid sensor has a detection range of 10-8 to 100 ppb, with a total width of 11 orders of magnitude. Moreover, the signal response of all concentrations is in the same current range, so the experiment is simple, and the detection can be carried out in a wide range without signal amplification. Under the optimal experimental conditions, it is also applicable to the actual sample detection. The design utilizes the recognition of aptamers of nucleic acids, It is also suitable for the detection of other targets with aptamer of nucleic acid. (2) Electrochemical nucleic acid sensors based on double gold enhancement for the detection of ochratoxin A using gold nanoparticles surface coupled nucleic acid probes for different electrode surfaces. Self-assembly to achieve the speed of OTA, Sensitive detection. The detection signal was enhanced by enrichment of more signal molecules by using the high specific surface area of gold nanoparticles, and the self-assembly of aptamer was increased by the gold nanoparticles assembled on the surface of the electrode. Thus, the detection width is improved. The detection range of the electrochemical aptamer is from 0.001 to 1000 ppb, which is two orders of magnitude wider than that of the base type. It can be applied to the detection of real samples, and can be applied to the detection of other targets by changing the aptamer sequence of nucleic acid.
【学位授予单位】：合肥工业大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：TS207.3;O657.1

【相似文献】