火龙果酵素中原花青素含量测定方法的建立

发布时间：2018-06-18 02:22

  本文选题：火龙果酵素 + 原花青素　； 参考：《中国酿造》2017年04期

【摘要】：采用香草醛-盐酸法对火龙果酵素中原花青素含量进行测定,考察反应温度、反应时间、香草醛质量浓度对溶液吸光度值的影响,并确定火龙果酵素中原花青素含量测定的方法。结果表明,原花青素含量的最适检测条件为1 mL火龙果酵素稀释液,5 mL 5 g/100 mL的香草醛甲醇溶液,4 mL 10%盐酸甲醇溶液,混匀后,35℃条件下显色反应20 min,在波长500 nm处测定吸光度值。精密度和重现性试验结果的相对标准偏差(RSD)值分别为1.23%和1.47%;平均回收率为99.5%~104.4%,RSD值为1.21%~2.11%。表明该法操作简便,精密度和重现性良好,准确性高,适用于火龙果酵素中原花青素含量的测定。
[Abstract]:The content of proanthocyanidins in the fermentins was determined by vanillin hydrochloric acid method. The effects of reaction temperature, reaction time and vanillin concentration on the absorbance of the solution were investigated. A method for the determination of procyanidin in fermentan was established. The results showed that the optimum conditions for the detection of procyanidins were as follows: 1 mL of floriculate dilution solution 5 mL 5 g / 100 mL of vanillin methanol solution 4 mL 10% methanol hydrochloride solution. After mixing at 35 鈩，

本文编号：2033582