基于温度响应型双亲水嵌段共聚物的荧光化学传感器的制备及其应用

发布时间：2018-06-18 03:35

本文选题：双亲水性嵌段共聚物 + 荧光化学传感器　；参考：《江苏大学》2017年硕士论文

【摘要】：近年来,自组装的概念已经被引入到荧光化学传感器的领域中,通过利用智能型聚合物作为响应的基体,制备新型荧光化学传感器能够实现多种功能的协同性以及更广泛的适应性。本课题利用了温度响应型双亲水性嵌段共聚物的自组装来设计和制备具有良好水溶性、温度响应性、金属离子或pH响应性、生物相容性、高灵敏性等集多种功能于的一体新型荧光化学传感器。(1)制备了一种新型基于双亲水性嵌段共聚物的金属离子荧光化学传感器,PEG113-b-P(NIPAM-co-SHMa)65,在温敏性嵌段中标记了金属离子识别基元SHMa。它可以作为金属离子和温度响应的多功能荧光化学传感器,不仅能够从众多金属离子中选择性识别Al3+和Zn2+,根据荧光颜色和荧光响应时间的不同来区别Al3+和Zn2+,而且具有很强的抗干扰能力、极佳的水溶性、较高的灵敏度和出色的生物相容性。一旦温度升高会导致温敏嵌段PNIPAM坍塌,DHBCs在水溶液中随即发生自组装,金属离子荧光响应基元SHMa将聚集到极性较小的疏水核里面,量子产率继而提高,检测灵敏度进一步提升。另外,回收率实验证明PEG113-b-P(NIPAM-co-SHMa)65可用于实际生物制品中Al3+或Zn2+的定性和定量检测。(2)制备了一种新型的基于双亲水性嵌段共聚物的pH荧光化学传感器,PEG113-b-P(NIPAM-co-SHMa-co-Rh6GEMa)62,并在温敏性嵌段中标记了碱性识别基元SHMa和酸性识别基元RhSAMa。它可以作为pH响应和温度响应的多功能荧光化学传感器,具有宽pH检测范围、温度响应性、水溶性、生物相容性、高灵敏度等特点。温度升高超过其LCST温度时,pH的检测信号可进一步增强。同时,通过自主开发的用于颜色识别的手机APP处理图像获取不同pH值下溶液的色差图及色差图数据ΔR,ΔG,ΔB。将pH 1-6和pH 8-14下溶液的ΔR,ΔG,ΔB数据分别利用SPSS 20建立主成份线性回归定量模型用于定量待测溶液的pH。最后,利用主成份线性回归定量模型开发了ColorExtract即时pH检测手机APP。(3)制备了两种新型基于双亲水性嵌段聚合物的金属离子荧光化学传感器,PEG113-b-P(NIPAM-co-BDMa)60,PEG113-b-P(NIPAM-co-R6GEMa)67,并分别在温敏性嵌段中标记Al3+和Fe3+识别基元BDMa和Rh6GEMa。它们组成的混合物可以作为温度和混合金属离子响应的多功能荧光化学传感器,而且具有很强的抗干扰能力、极佳的水溶性、较高的灵敏度和出色的生物相容性。此外,温度诱导嵌段坍塌后,形成以P(NIPAM-co-BDMa)和P(NIPAM-co-R6GEMa)为核的混合胶束,可以进一步提高其对Al3+和Fe3+的检测灵敏度。同时,本章通过自主开发的用于颜色识别的手机APP处理图像以获取不同Al3+和Fe3+混合比例下溶液反应前后的色差图及色差图数据ΔR,ΔG,ΔB,利用SPSS 20建立了对Al3+和Fe3+混合金属离子比例定量检测的主成份线性回归模型并对模型进行了验证。
[Abstract]:In recent years, the concept of self-assembly has been introduced into the field of fluorescent chemical sensors by using intelligent polymers as the responsive matrix. The novel fluorescent chemical sensor can achieve multi-functional cooperation and wider adaptability. In this paper, the self-assembly of thermo-responsive dihydrophilic block copolymers was used to design and prepare water-soluble, temperature-responsive, metal or pH responsive, biocompatible, and water-soluble dihydrophilic block copolymers. A novel metal ion fluorescence chemical sensor based on dihydrophilic block copolymers PEG113-b-PNPAM-co-SHMa65 was prepared. The metal ion recognition unit SHMaa was labeled in the thermosensitive block. It can be used as a multifunctional fluorescent chemical sensor for metal ion and temperature response. It not only can selectively recognize Al3 and Zn2 from many metal ions, but also can distinguish Al3 and Zn2 according to the difference of fluorescence color and fluorescence response time. And has very strong anti-interference ability, excellent water-soluble, high sensitivity and excellent biocompatibility. Once the temperature increases, the temperature sensitive block PNIPAM collapses and the DHBCs self-assemble in aqueous solution, and the metal ion fluorescence response unit SHMa will gather in the hydrophobic nucleus with small polarity, and the quantum yield will increase, and the detection sensitivity will be further improved. In addition, A novel pH fluorescence chemical sensor based on dihydrophilic block copolymers, PEG113-b-PNPAM-co-Rh6GEMa62, was prepared by using PEG113-b-PNNIPAM-co-SHMa-65 for the qualitative and quantitative detection of Al3 or Zn2 in practical biological products. A novel pH fluorescence chemical sensor based on dihydrophilic block copolymers, PEG113-b-PNNIPAM-co-SHMa-co-Rh6GEMa62, was prepared and labeled in thermosensitive block. Basic recognition unit SHMa and acid recognition unit RhSAMa. It can be used as a multifunctional fluorescent chemical sensor for pH response and temperature response. It has the characteristics of wide pH detection range, temperature response, water solubility, biocompatibility, high sensitivity and so on. The detection signal of pH value can be further enhanced when the temperature is higher than its LCST temperature. At the same time, the color difference map and color difference map data 螖 R, 螖 G, 螖 B of the solution at different pH values were obtained by the mobile phone app developed by ourselves. Using the data of 螖 R, 螖 G, 螖 B at pH 1-6 and pH 8-14, respectively, the principal component linear regression quantitative model was established by SPSS20 to quantify the pHs of the solution to be measured. Last Two novel metal ion fluorescence chemical sensors based on bihydrophilic block polymer, PEG113-b-PniPAM-co-BDMaC60 / PEG113-b-PNNIPAM-co-R6GEMa67 were developed by using a quantitative model of principal component linear regression (PCA) for real-time pH detection of ColorExtract. Two novel metal ion fluorescence sensors (PEG113-b-PNPAM-co-R6GEMa67) were prepared, respectively, and labeled Al3 and PNIPAM-co-R6GEMa67 in thermosensitive blocks. Fe3 recognized the elements BDMa and Rh6GE Ma. The mixture can be used as a multifunctional fluorescent chemical sensor for temperature and mixed metal ion response, and has strong anti-interference ability, excellent water solubility, high sensitivity and excellent biocompatibility. In addition, the temperature induced block collapse resulted in the formation of mixed micelles with PNIPAM-co-BDMaand PNNIPAM-co-R6GEMa. which can further improve the sensitivity to Al3 and Fe3 detection. meanwhile In this chapter, a mobile phone app for color recognition is developed to process images to obtain chromatic aberration map and chromatic aberration map data 螖 R, 螖 G, 螖 B before and after solution reaction under different Al3 and Fe 3 mixed ratios. Using SPSS20, the mixed metal ions of Al3 and Fe 3 are established. The principal component linear regression model of proportional quantitative detection was used to verify the model.
【学位授予单位】：江苏大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：O657.3

【参考文献】