利用IR光谱原位检测生物酶活性的二维相关分析校正

发布时间：2018-06-20 05:09

本文选题：IR光谱 + 原位检测　；参考：《光谱学与光谱分析》2016年S1期

【摘要】：在利用IR光谱原位监测酶促反应的过程中,反应物与底物红外特征吸收峰会随反应进程发生变化,因此可根据单位时间内特征峰吸收值的变化量来反映酶的催化效率。然而,该方法在实际应用过程中还存在诸多的局限性有待改进。例如,峰位选取不准确和谱带严重重叠无法分辨/分离等都将给酶活力的测试结果带来偏差。二维相关分析与IR光谱的结合使用将在一定程度上改进上述问题,我们将在本文中以具体事例分别阐述。
[Abstract]:In the process of in situ monitoring the enzymatic reaction by IR spectroscopy, the infrared characteristic absorption summit of reactants and substrates changes with the reaction process, so the catalytic efficiency of the enzyme can be reflected according to the change of the absorption value of the characteristic peak in unit time. However, there are still many limitations in the practical application of this method. For example, the inaccuracy of peak selection and the serious overlap of spectrum bands can not distinguish / separate the enzyme activity, which will bring deviation to the test results of enzyme activity. The combination of two dimensional correlation analysis and IR spectra will improve the above problems to some extent. We will give some examples in this paper.
【作者单位】：吉林大学超分子结构与材料国家重点实验室;
【基金】：国家自然科学基金项目(21373101,91027027)资助
【分类号】：Q55;O657.33

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