基于磁性纳米粒子自组装的制备及荧光传感应用

发布时间：2018-06-24 13:13

本文选题：球形羟基氧化铁 + 量子点　；参考：《安徽师范大学》2017年硕士论文

【摘要】：我们通过不同合成条件的控制,借助纳米粒子的自组装,制备出尺寸可调的球形羟基氧化铁(Fe OOH)超级纳米粒子。此外,荧光探针由于其好的灵敏性、简单性以及多功能性,在生物医疗实验、疾病标志物的诊断和有毒物质的检测方面,被广泛应用。借助磁性纳米粒子的自组装,结合磁性分离及等离子体增强荧光,以量子点为信号输出,构建了一种从完全的“零背景”到“真实信号放大”的传感模式,这种模式打破了传统的“turn-on”模式的上限与下限。对于分析物透明质酸酶的检测,相比于传统的荧光模式,其灵敏度提高了10~4-10~6倍。论文主要包括以下四个章节:第一章为绪论,主要综述了FeOOH纳米材料、半导体纳米材料以及磁性材料的概述、合成以及应用进展。并提出了待解决的问题和潜在应用,基于此提出了本论文的工作设想。第二章通过回流时间、预先p H值以及原料的量的控制,成功制备出不同尺寸的球形Fe OOH超级纳米粒子。第三章利用量子点(QDs)、磁性粒子(Fe_3O_4 NPs)以及贵金属纳米粒子(Ag NPs)的易于修饰和功能化的特点,基于磁性分离与等离子体增强效应,实现从零背景到信号放大的超“turn-on”荧光传感模式。以量子点为信号输出,高灵敏度地检测了尿液和血清中的癌症标志物、水解酶---透明质酸酶。第四章为本篇论文的创新之处以及工作展望。对已经开展的工作进行了总结,并展望了后续的研究工作。
[Abstract]:The spherical ferric hydroxide (FeOOH) superparticles with adjustable size were prepared by the control of different synthesis conditions and self-assembly of nanoparticles. In addition, fluorescent probes are widely used in biomedical experiments, diagnosis of disease markers and detection of toxic substances due to their good sensitivity, simplicity and versatility. With the help of self-assembly of magnetic nanoparticles, combined with magnetic separation and plasma-enhanced fluorescence, a sensing mode from "zero background" to "real signal amplification" was constructed by using quantum dots as signal output. This model breaks the upper and lower limits of the traditional "turn-on" model. The sensitivity of hyaluronidase was 104-106 times higher than that of traditional fluorescence mode. The thesis mainly includes four chapters as follows: the first chapter is the introduction, which mainly summarizes the progress of FeOOH nanomaterials, semiconductor nanomaterials and magnetic materials, synthesis and application of FeOOH nanomaterials. The problems to be solved and the potential applications are put forward, and based on this, the working assumption of this paper is put forward. In the second chapter, the spherical FeOOH superparticles with different sizes were successfully prepared by controlling the reflux time, the pre-pH value and the amount of raw materials. In chapter 3, based on magnetic separation and plasma-enhanced effect, the properties of quantum dots (QDs), magnetic particles (FeSs) and noble metal nanoparticles (Ag NPs) are easily modified and functionalized. The super-" turn-on "fluorescence sensing mode from zero background to signal amplification is realized. Using quantum dots as signal output, the cancer marker, hydrolase-hyaluronidase, in urine and serum was detected with high sensitivity. The fourth chapter is the innovation of this paper and work prospects. The work has been summarized, and the future research work is prospected.
【学位授予单位】：安徽师范大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：O657.3;TB383.1

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