人中黄的HPLC指纹图谱及炮制机理初探

发布时间：2018-06-26 04:00

本文选题：人中黄 + HPLC　；参考：《中药材》2017年05期

【摘要】：目的:建立中药人中黄HPLC指纹图谱测定方法,为其质量评价提供参考。指认其中主要成分,并对生制品进行化学成分比较,探析人中黄的炮制机制,为创立新的科学制备方法奠定基础。方法:HPLC法建立中药人中黄指纹图谱,对照品确定有效成分,"中药色谱指纹图谱相似度评价系统(A版)"分析不同样品图谱的相似度,镜像图谱对比分析人中黄共有模式图与甘草指纹图谱的区别。结果:对12批市售人中黄药材进行相似度评价,标定了18个共有峰;市售人中黄与共有模式图谱的相似度为0.8~0.9。自制样品与共有模式图谱的相似度均小于0.9。标定出6种成分,5个共有色谱峰分别是甘草酸、甘草素、异甘草素、甘草查尔酮A和甘草次酸,1个非共有峰甘草苷。方法学考察结果符合指纹图谱要求,稳定良好。人中黄指纹图谱与甘草指纹图谱镜像对比结果证明人中黄化学成分发生较大变化,差异性明显。结论:建立了人中黄的HPLC指纹图谱,相似度高,精密度、稳定性、重复性均表现良好,揭示人中黄与甘草相比化学成分在炮制过程中的变化趋势,为人中黄药材质量控制标准提供参考。
[Abstract]:Objective: to establish a method for the determination of HPLC fingerprint of Chinese herbal medicine Zhonghuanhuang, and to provide reference for its quality evaluation. The main components were identified, and the chemical constituents of raw products were compared to explore the processing mechanism of human yellow in order to lay a foundation for the establishment of a new scientific preparation method. Methods the yellow fingerprint of Chinese traditional medicine was established by HPLC, and the effective components were determined. The similarity evaluation system of chromatographic fingerprint of traditional Chinese medicine (version A) was used to analyze the similarity of different samples. The difference between yellow common pattern map and Glycyrrhiza uralensis fingerprint was analyzed. Results: the similarity of 12 batches of Chinese yellow herbs on the market was evaluated, and 18 common peaks were calibrated, and the similarity between yellow and common pattern was 0.80.9.Results: the results showed that there were 18 common peaks in 12 batches of Chinese herbal medicines sold on the market. The similarity between the self-made samples and the shared patterns was less than 0. 9. Six components were identified, and five common chromatographic peaks were glycyrrhizic acid, glycyrrhizin, isoglycyrrhizin, glycyrrhizin A and glycyrrhetinic acid, and one non-common peak glycyrrhizin. The results of methodological investigation accord with the requirements of fingerprint and are stable. The results of comparison between yellow fingerprint and glycyrrhiza fingerprint showed that the chemical composition of yellow in human was changed greatly and the difference was obvious. Conclusion: the HPLC fingerprint of human yellow was established, and the similarity, precision, stability and repeatability were all good, which revealed the changing trend of chemical constituents between human yellow and Glycyrrhiza uralensis in the process of processing. To provide a reference for the quality control standard of Chinese herbal medicine.
【作者单位】：河南中医药大学;
【基金】：国家中医药管理局中药炮制技术传承基地建设项目(2100601中医(民族医)专项) 河南中医药大学研究生科研苗圃工程项目(MPYJS2017-06)
【分类号】：O657.72;R284.1

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