QuEChERS结合超高效液相色谱-串联质谱法同时测定番茄中噻虫嗪、噻虫胺、螺虫乙酯及其代谢物残留

发布时间：2018-06-27 04:56

  本文选题：QuEChERS法 + 超高效液相色谱-串联质谱法　； 参考：《分析测试学报》2017年12期

【摘要】：建立了QuEChERS-UPLC-MS/MS法快速同时检测番茄中噻虫嗪及其代谢产物(噻虫胺)、螺虫乙酯及其4种代谢产物(BYI08330-enol-glucoside、BYI08330-mono-hydroxy、BYI08330-enol和BYI08330-ketohydroxy)残留的分析方法。样品经乙腈提取,NaCl和无水Mg SO4除水后,经N-丙基乙二胺(PSA)和C18粉末净化,用超高效液相色谱-串联质谱(UPLC-MS/MS)分析,采用多反应离子监测模式(MRM)检测,基质匹配标准溶液外标法定量。7种目标物质在0.2~2 000μg/L质量浓度范围内均具有良好的线性关系(r2≥0.999 2),在0.002、0.02、0.20、2.00 mg/kg加标水平下的平均回收率为79.9%~104%,相对标准偏差(RSD)为0.94%~6.4%,方法定量下限(LOQ)为0.002 mg/kg。该方法快速简便、灵敏度高、重现性好,能满足番茄中噻虫嗪、噻虫胺和螺虫乙酯及其代谢产物残留的快速检测和确证要求。
[Abstract]:A QuEChERS-UPLC-MS / MS method was established for the rapid and simultaneous determination of thiamethoxin and its metabolites (thiamamine), ethyl spirochthyl and its four metabolites (BYI08330-mono-hydroxypyridol and BYI08330-ketohydroxy) in tomato. The method was used to determine the residues of thiamethazine and its metabolites (thiamethylamine), BYI08330-enol-glucoside (BYI08330-enol and BYI08330-ketohydroxy). The samples were purified by N propyl ethylenediamine (PSA) and C18 powder after removing water by acetonitrile extraction of sodium chloride and anhydrous mg so 4. The samples were analyzed by UPLC-MS / MS and detected by multi-reaction ion monitoring mode (MRM). The matrix matching standard solution external standard solution method was used to quantify 7 target substances in the range of 0.2 ~ 2 000 渭 g / L mass concentration (r _ 2 鈮，

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