中药白芷中欧前胡素、异欧前胡素体外经皮渗透特性的串联质谱研究

发布时间：2018-06-27 22:28

本文选题：高效液相色谱-串联质谱(HPLC-MS/MS) + 白芷　；参考：《质谱学报》2017年01期

【摘要】：欧前胡素和异欧前胡素属于6,7-呋喃香豆素类化合物,是中药白芷的主要药效成分。为了考察二者的经皮渗透特性,采用改进型的Franz扩散池,以大鼠离体皮肤作为渗透屏障,以高效液相色谱-串联质谱(HPLC-MS/MS)法测定白芷提取液的经皮渗透液中欧前胡素和异欧前胡素的含量,据此来考察白芷提取液中欧前胡素和异欧前胡素的经皮渗透特性。实验结果表明,欧前胡素和异欧前胡素在48h内,单位面积的累积渗透量分别为116.11μg/cm~2和140.84μg/cm~2,累积渗透百分率分别为37.75%和71.86%。欧前胡素的稳态渗透速率为26.5μg/h·cm~2,其体外经皮渗透符合Higuchi方程。本研究阐明了欧前胡素的体外经皮渗透机制,可为白芷作为外用经皮给药制剂的发展提供实验依据和理论基础。
[Abstract]:Ephedronin and isophorin belong to 6-7-furancoumarin compounds and are the main active components of Radix angelicae dahuricae (Radix angelicae dahuricae). In order to investigate the percutaneous permeation characteristics of the two cells, an improved Franz diffusion cell was used, and the isolated rat skin was used as the permeation barrier. High performance liquid chromatography-tandem mass spectrometry (HPLC-MS / MS) was used to determine the transdermal pericarpitin and isoeuranthin of Angelica dahurica extract. The results showed that the cumulative osmotic capacity per unit area was 116.11 渭 g/cm~2 and 140.84 渭 g / cm ~ (-2), respectively, and the cumulative osmotic percentages were 37.75% and 71.86%, respectively. The steady-state osmotic rate was 26.5 渭 g / h 路cm ~ (-2), and the percutaneous permeation in vitro was in accordance with Higuchi equation. In this study, the mechanism of transdermal permeation of uranine in vitro was elucidated, which could provide experimental basis and theoretical basis for the development of Angelica dahurica as an external transdermal drug delivery agent.
【作者单位】：吉林大学药学院;中国科学院长春应用化学研究所长春质谱中心吉林省中药化学与质谱重点实验室;吉林农业大学;吉林省现代中药及生物制药基地建设办公室;
【基金】：吉林省科技发展计划项目(20140311096YY)资助
【分类号】：R285.5;O657.63

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