UPLC Orbitrap HRMS法分析西洋参蒸参弃液浓缩物中皂苷类成分

发布时间：2018-07-01 11:02

本文选题：超高效液相色谱-四极杆静电场轨道阱高分辨质谱(UPLC + Orbitrap　；参考：《质谱学报》2017年01期

【摘要】：采用超高效液相色谱-四极杆静电场轨道阱高分辨质谱(UPLC Orbitrap HRMS)技术测定西洋参蒸参弃液浓缩物中15种人参皂苷单体成分含量。采用Thermo Scientific Syncronis C18色谱柱(100mm×2.1mm×1.7μm),以乙腈-0.1%甲酸水溶液为流动相,使用电喷雾电离源(ESI),四极杆静电场轨道阱质量分析器,高分辨质谱仪采集数据。结果表明,人参皂苷Rg1、Rg2、Rg3、Rb1、Rb2、Rc、Rd、Re、Rh1、Rh2、Rk1、Ro、F1、F2和伪人参皂苷F11在线性范围内均存在良好的线性关系,48h内稳定性实验RSD值均小于5%,平均加样回收率为94.38%~102.10%,RSD值不大于3%。西洋鲜参在不同蒸制温度和蒸制时间下得到9组蒸参弃液,通过分析其浓缩物中15种人参皂苷单体化合物的含量,并比较各成分含量的变化情况,总结了4种类型人参皂苷的化学转化规律。该方法简便、准确、灵敏度高、专属性强、重复性好,适用于西洋参蒸参弃液浓缩物中皂苷成分含量的测定,可为开发蒸参弃液提供有效的检测手段。
[Abstract]:Ultrahigh performance liquid chromatography-quadrupole electrostatic field trap high resolution mass spectrometry (UPLC Orbitrap HRMS) was used to determine the contents of 15 ginsenoside monomers in Panax quinquefolium. A Thermo Scientific Syncronis C18 column (100mm 脳 2.1mm 脳 1.7 渭 m),) with acetonitrile-0.1% formic acid solution as mobile phase was used to collect data by electrospray ionization source (ESI), quadrupole electrostatic field trap mass analyzer and high resolution mass spectrometer. The results showed that there was a good linear relationship between Rg1, Rg2, Rg3, Rb1 and Rb2, and pseudoginsenoside F11 in the linear range, and the average recovery was 94.38102.100.The average recovery was 94.38102.10. Nine groups of distilled discards were obtained from fresh ginseng at different evaporation temperature and time. The contents of 15 kinds of ginsenoside monomers in its concentrate were analyzed, and the changes of the contents of each component were compared. The chemical transformation of four types of ginsenosides was summarized. The method is simple, accurate, sensitive, specific and reproducible. It is suitable for the determination of saponins in American ginseng evapotranspiration liquid concentrate and can provide an effective method for the development of evapotranspiration solution.
【作者单位】：长春中医药大学吉林省人参科学研究院;中国科学院长春应用化学研究所长春质谱中心;
【基金】：国家自然科学基金面上项目(21475012) 吉林省科技发展计划项目(20160520181JH) 吉林省教育厅"十三五"科学技术研究项目(吉教科合字[2016]第30号) 公益性行业(农业)科研专项经费项目(20130311106)资助
【分类号】：R284.1;O657.63

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