液体农药制剂中烷基酚及其聚氧乙烯醚类助剂的高效液相色谱分析

发布时间：2018-07-06 09:07

本文选题：农药助剂 + 液体农药制剂　；参考：《农药学学报》2017年02期

【摘要】：建立了一种测定6类液体农药制剂中烷基酚(alkylphenols,APs)及其聚氧乙烯醚类(alkylphenol ethoxylates,APEOs)助剂的高效液相色谱-荧光分析方法。乳油、水剂、水乳剂、悬浮剂、可溶液剂和微乳剂样品均经甲醇超声辅助提取,稀释后过聚丙烯膜,采用Poroshell 120EC-C_(18)(4.6 mm×100 mm,2.7μm)分离,V(甲醇):V(乙腈):V(水)=75.5:6.5:18.0三元流动相等度洗脱,荧光检测器检测,外标法定量。结果表明:在0.05~10或0.1~20mg/L范围内,APs和APEOs的峰面积与其质量浓度间呈良好线性关系(R~2均大于0.999 8);在添加水平范围内(APs:0.5、2、5 mg/g;APEOs:1、5、25、50mg/g),6类液体农药剂型中APs和APEOs的平均回收率为73%~110%,相对标准偏差为0.6%~14.9%,方法的定量限在0.5~1mg/g。该方法简单、快速、准确,适用于不同液体农药制剂中烷基酚及其聚氧乙烯醚类助剂的测定。
[Abstract]:A high performance liquid chromatography-fluorescence analysis method for the determination of alkylphenols (APs) and alkylphenol ethoxylatesl (APEOs) promoters in six kinds of liquid pesticide formulations was established. Emulsion, water agent, emulsion, suspending agent, soluble agent and microemulsion were extracted by methanol ultrasonic assisted extraction, diluted over polypropylene membrane, separated by Poroshell 120EC-C18 (4.6 mm 脳 100mm 2.7 渭 m) and V (methanol): v (acetonitrile): v (water) 75.5: 6.5: 18.0. Fluorescence detector detection, external standard quantitative analysis. The results showed that there was a good linear relationship between the peak area of APs and APEOs in the range of 0.05g / L or 0.1g / L (R2 > 0.999), and the average recovery of APs and APEOs in six kinds of liquid pesticide formulations (APs: 0.5 mg / L) was 73110, and the average recovery of APEOs was 73110 in the range of APs: 0.5 mg / L (APs: 0.5 mg / L). The standard deviation was 0.6 and the quantitative limit of the method was 0.5 mg / g. The method is simple, rapid and accurate. It is suitable for the determination of alkylphenol and polyoxyethylene ether in different liquid pesticide formulations.
【作者单位】：中国农业科学院农业质量标准与检测技术研究所农业部农产品质量安全重点实验室;
【基金】：科技部科技基础性工作专项项目(2013FY110100) 中国农业科学院科技创新工程“农业化学污染物残留检测及行为研究”创新团队
【分类号】：TQ450.7;O657.72

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