基于UPLC-Q-TOF-MS技术的广地龙血清药物化学初步研究

发布时间：2018-07-09 20:14

  本文选题：广地龙 + 血清药物化学　； 参考：《中药材》2017年04期

【摘要】：目的:对广地龙的血清药物化学进行初步研究。方法:应用超高液相色谱-串联四级杆飞行时间质谱技术(UPLC-Q-TOF-MS)建立广地龙及其含药血清样品的分析方法;比较体外样品、空白血清及含药血清分析结果,结合Peakview 2.0和Metabolite Pilot 1.5数据分析软件鉴定和表征广地龙血中移行成分。结果:从大鼠含药血清中发现了13个移行成分,包括10个原型成分和3个代谢产物。结论:初步确定了广地龙的血中原型成分及代谢产物,有助于阐明其药效物质基础和作用机理。
[Abstract]:Objective: to study the serum pharmacochemistry of Guandelong. Methods: UPLC-Q-TOF-MS was used to establish a method for the analysis of Guandelong and its drug-containing serum samples, and to compare the analysis results of in vitro samples, blank serum and drug-containing serum. Peakview 2.0 and Metabolite pilot 1.5 data analysis software were used to identify and characterize the transitory components in the blood of Dioscorea grandii. Results: thirteen transitory components including 10 prototypes and 3 metabolites were found in rat serum containing drugs. Conclusion: the prototypes and metabolites in the blood of Dioscorea grandifolia were preliminarily determined, which was helpful to elucidate the substance basis and mechanism of its pharmacodynamics.
【作者单位】： 黑龙江省中医药科学院;
【基金】：国家自然科学基金(81374045) 黑龙江省青年基金(QC2011C114) 黑龙江省应用技术研究与开发计划(PB15F005)
【分类号】：O657.63;R284.1
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本文编号：2110527