壳聚糖氧化降解制备聚合度6以下的壳寡糖

发布时间：2018-07-11 10:15

  本文选题：壳聚糖 + 壳寡糖　； 参考：《现代食品科技》2017年09期

【摘要】：壳寡糖是壳聚糖经降解后的低聚物,其相对分子质量较小,水溶性好,易于吸收,生物活性高。本实验采用过氧化氢氧化降解脱乙酰度高于95%的壳聚糖,利用离子色谱-脉冲安培法对低聚合度的壳寡糖进行定性和定量分析,以制备聚合度在6以下的壳寡糖。以降解产物的离子色谱中聚合度6以下的峰面积和与产物得率之积作为响应指标,并结合凝胶渗透色谱来考察过氧化氢浓度、反应时间、反应温度对壳聚糖降解的影响及降解特性,并利用响应面分析法对氧化条件进行优化。研究结果表明,采用离子色谱测定聚合度6以下的壳寡糖在方法学上是可行的,具有良好的精密度、稳定性和重现性。得到的最佳降解工艺条件为:过氧化氢浓度4.50%、反应时间6 h、反应温度56℃。由最优条件下的降解产物离子色谱图可知,聚合度越低的壳寡糖越容易得到;可利用离子色谱法对壳聚糖的降解过程进行调控。
[Abstract]:Chitosan oligosaccharides are low molecular weight, water soluble, easy to absorb and high bioactivity. The chitosan whose degree of deacetylation is more than 95% was oxidized by hydrogen peroxide. The chitosan with low degree of polymerization was prepared by ion chromatography-pulse amperometric method. The peak area of polymerization degree below 6 and the product of product yield were used as the response index, and the concentration of hydrogen peroxide and reaction time were investigated by gel permeation chromatography. The effect of reaction temperature on the degradation of chitosan and its degradation characteristics were studied. The oxidation conditions were optimized by response surface analysis (RSM). The results show that the determination of chitosan oligosaccharides with polymerization degree below 6 by ion chromatography is feasible in methodology and has good precision, stability and reproducibility. The optimum degradation conditions are as follows: hydrogen peroxide concentration 4.50, reaction time 6 h, reaction temperature 56 鈩，

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