应用纳米球刻蚀法在自组装膜修饰的硅表面生成中尺度的网状蛋白层（英文）

发布时间：2018-07-11 12:19

本文选题：纳米球刻蚀法 + 溶菌酶　；参考：《物理化学学报》2017年04期

【摘要】：在自组装膜修饰的硅表面制备有序的蛋白阵列是研发生物传感器的先决条件之一,因此如何产生有序的表面蛋白阵列一直是生物医药研究方向的前沿。本研究通过应用纳米球刻蚀法在氧化的10-烯基十一烷基三氯硅烷自组装膜修饰的硅表面生成了网状结构溶菌酶蛋白层。网孔的大小(从纳米到微米级别)由表面沉积的纳米球的尺寸来调控。我们利用原子力显微镜和荧光显微镜对样品表面进行了详细表征。结果表明:这种新方法比传统的通过扫描探针在固体表面修饰而聚集溶菌酶蛋白的方法更快捷简便,而且它能够在相对大的硅表面形成网状蛋白层。此外,网孔表面附着具有强吸附活性的羧酸基团层,它可以通过静电吸引或者共价结合来吸附液相中的第二种蛋白分子。
[Abstract]:The preparation of ordered protein arrays on the surface of a self assembled monolayer is one of the prerequisites for the development of biosensors. Therefore, how to produce an ordered surface protein array has been the forefront of biomedical research. This study modified the 10- alkyl eleven alkyl three chlorosilane self assembled membrane by the application of nanospheres etching. The surface of the reticular lysozyme protein was formed on the surface of the silicon. The size of the mesh (from nanoscale to micron) was regulated by the size of the surface deposited nanospheres. We used atomic force and fluorescence microscopy to characterize the surface of the sample. The results showed that the new method was repaired on the solid surface by the traditional scanning probe. The method of assembling the lysozyme protein is quicker and simpler, and it can form a reticular protein layer on a relatively large silicon surface. In addition, the surface of the mesh is attached with a strongly adsorbed carboxylic acid group layer, which can adsorb second proteins in the liquid phase by electrostatic attraction or covalent binding.
【作者单位】： Department
【基金】：supported by National Institute of General Medical Sciences of the National Institutes of Health,USA(P20GM103436) National Science Foundation,USA(3048111570-15-153)~~
【分类号】：TB383.1;O629.73

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