荧光增强型金属纳米簇的生物检测研究

发布时间：2018-07-12 18:30

本文选题：荧光金纳米簇 + 生物检测　；参考：《东南大学》2017年硕士论文

【摘要】：近些年来,在催化,传感等领域,金属纳米材料被证实有着广泛的应用。科学家们发现,腺嘌呤及其衍生物可以被用来合成纳米颗粒,然而利用它们合成的配合物、聚合物和纳米颗粒之中,仅仅有非常少的一部分产物可以产生荧光。在本论文中,我们选取了金纳米材料与腺苷来合成AuAXP(单磷酸腺苷、二磷酸腺苷、三磷酸腺苷)作为实验研究基础。首先,将腺苷和金纳米颗粒结合,观察所得到的产物各自的荧光强度。然后进行对照实验,比较在不同添加物影响下产物的荧光增强效应,比如加入钙、镁、锌等离子会否发生增强作用,并且考察观察在荧光增强作用下,原本的产物在细胞成像方面的表现有什么变化。本课题主要对荧光增强型金纳米簇的制备和表征,以及其在细胞和生物分子中的检测和成像进行了研究,主要利用荧光光谱仪、共聚焦荧光显微镜、紫外光谱仪以及酶标仪等手段重点研究了不同荧光金纳米簇在细胞和生物分子检测和生物成像中的作用,并对其潜在的应用前景进行展望。本实验选取了氯金酸溶液作为反应前驱体,柠檬酸作为还原剂和保护剂,探究了在不同的条件下例如不同浓度,不同温度的反应前驱体,对合成产物纳米金簇的影响。并从结果之中对比挑选了最优的方案,成功制备了荧光金纳米簇。本实验的这种合成方法操作简便,实验所需成本相对较低,所合成的金纳米簇具有低荧光性、荧光寿命长、颗粒粒径小且粒径分布十分均一、生物相容性好无毒性等等优点,且在腺苷酸衍生物上,具有许多的能与金属离子结合的位点,极为有效的增强了荧光强度。在本论文中,我们通过考察了不同金属离子对合成的荧光金属复合物的荧光增强效应和生物成像能力,不仅仅证明了荧光增强型金纳米簇在生物检测,细胞成像等等方面都有着有效的应用,还为如何提高检测的精确度,成像清晰度等至关重要的要素提供了一些不同的研究思路。
[Abstract]:In recent years, metal nanomaterials have been widely used in catalysis, sensing and other fields. Scientists have found that adenine and its derivatives can be used to synthesize nanoparticles, but very few of the complexes, polymers and nanoparticles they synthesize produce fluorescence. In this thesis, we selected gold nanomaterials and adenosine to synthesize AuAXP (adenosine monophosphate, adenosine diphosphate, adenosine triphosphate) as the experimental basis. Firstly, the fluorescence intensity of the product was observed by combining adenosine with gold nanoparticles. Then the contrast experiment was conducted to compare the fluorescence enhancement effect of the product under the influence of different additives, such as calcium, magnesium and zinc plasma, and to observe the effect of fluorescence enhancement. What changes have been made in the performance of the original product in cellular imaging. In this paper, the preparation and characterization of fluorescent enhanced gold nanoclusters and their detection and imaging in cells and biomolecules were studied, mainly using fluorescence spectrometer and confocal fluorescence microscope. The role of different fluorescent gold nanoclusters in cell and biomolecular detection and biomolocation was studied by means of UV spectrometer and enzyme labeling. The potential applications of these clusters were also prospected. In this experiment, chlorgold acid solution was selected as the reaction precursor, citric acid as reducing agent and protective agent. The effects of different concentration and temperature of reaction precursor on the synthesis of nano-gold clusters were investigated. The optimal scheme was selected from the results, and the fluorescent gold nanoclusters were successfully prepared. The synthesized gold nanoclusters have the advantages of low fluorescence, long fluorescence life, small particle size and uniform particle size distribution, good biocompatibility, etc. In addition, adenylic acid derivatives have many sites that can bind to metal ions, which can effectively enhance the fluorescence intensity. In this thesis, we investigated the fluorescence enhancement effect and the biological imaging ability of the fluorescent metal complexes synthesized by different metal ions, which not only proved that the fluorescence enhanced gold nanoclusters were in the biological detection. Cell imaging and other aspects of effective applications, but also for how to improve the accuracy of detection, imaging clarity and other crucial elements to provide some different research ideas.
【学位授予单位】：东南大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：O657.3;R318

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