波长切换高效液相色谱法同时测定青翘抗毒颗粒中7个成分的含量

发布时间：2018-07-14 11:46

【摘要】：目的:建立高效液相色谱法同时测定青翘抗毒颗粒中绿原酸、马钱苷、连翘苷、葛根素、(R,S)-告依春、射干苷、咖啡酸的含量。方法:采用岛津InertSustain C18柱(250 mm×4.6 mm,5μm),以甲醇-0.3%磷酸水溶液为流动相进行梯度洗脱;流速为1.0mL·min-1;进样量为5μL;检测波长为240 nm(0~25 min,检测(R,S)-告依春)、327 nm(25~39 min,检测绿原酸和咖啡酸)、240 nm(39~45 min,检测葛根素和马钱苷)、265 nm(45~53 nm,检测射干苷)、228 nm(53~65 min,检测连翘苷);柱温为30℃。结果:绿原酸、马钱苷、连翘苷、葛根素、射干苷、咖啡酸、(R,S)-告依春7个成分的进样质量浓度分别在4.56~228μg·mL-1(R2=0.999 9)、4.04~202μg·mL-1(R2=0.999 8)、8.10~405μg·mL-1(R2=0.999 9)、8.06~161μg·mL-1(R2=0.999 9)、2.10~105μg·mL-1(R2=0.999 9)、3.46~173μg·mL-1(R2=0.999 9)、4.58~229μg·mL-1(R2=0.999 9)与峰面积呈良好的线性关系;各组分分离度良好;加样回收率(n=6)分别为99.35%(RSD为2.6%)、100.76%(RSD为1.5%)、100.34%(RSD为0.9%)、100.63%(RSD为1.5%)、100.31%(RSD为1.7%)、100.70%(RSD为1.6%)、99.03%(RSD为1.7%)。结论:本研究建立的含量测定方法符合方法学验证要求,可用于青翘抗毒颗粒7个指标性成分的同时测定。
[Abstract]:Objective: to establish a high performance liquid chromatography (HPLC) method for the simultaneous determination of Lv Yuan acid, marganin, suspensin, puerarin, (RHs)-Gouyichun, etoside and caffeic acid in Qingqiao Kangdu granules. Methods: the gradient elution was carried out on InertSustain C18 column (250mm 脳 4.6mm ~ 5 渭 m),) with methanol-0.3% phosphoric acid solution as mobile phase. The flow rate was 1.0 mL min-1, the amount of sample was 5 渭 L, the detection wavelength was 240nm (0 ~ 25 min), the detection wavelength was (RNS) -Gouyichun (2539 min), the Lv Yuan acid and caffeic acid were detected at 240nm (3945 min), the puerarin and marganin were detected at 265 nm (4553 nm), and the column temperature was 30 鈩，

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