碳基复合纳米材料构建新型适配体传感器检测抗生素
发布时间：2018-07-18 10:29
【摘要】：抗生素被普遍应用于治疗动物和人类的疾病,同时也被作为促生长因子应用于畜牧领域。而抗生素使用不当及过度使用会造成在生态系统中及动物源性食品中抗生素的残留。由于其对公众健康和生态系统的威胁,抗生素残留问题已经引起了越来越广泛的关注。因此,开发高效、灵敏的抗生素检测方法是十分必要的。本文将先进的纳米技术和适配体传感器技术结合起来,制备了三种性能优良的电化学适配体传感器,分别实现了对青霉素、氨苄西林、氯霉素的高效、灵敏检测。本文主要内容如下:1.基于PEDOT-AuNPs/GR-Fe_3O_4NPs复合材料构建电化学适配体传感器检测青霉素:以青霉素适配体为生物识别元件,以磁性石墨烯纳米复合材料(GR-Fe_3O_4NPs)和聚3,4-乙撑二氧噻吩-金纳米粒子(PEDOT-AuNPs)复合材料为基底,制备了一种新型的电化学适配体传感器来灵敏地检测青霉素。复合材料的结构通过扫描电子显微镜(SEM)来研究,所制备的传感器的电化学性能通过循环伏安法(CV)、电化学阻抗(EIS)和差分脉冲伏安法(DPV)来研究。在最优条件下,该传感器具有较高的灵敏度,线性范围为0.1-200 ng/mL,检测限为0.057 ng/mL。2.基于AuNPs@MGR/PEDOT-AuNPs复合材料构建电化学适配体传感器检测氨苄西林:以氨苄西林适配体为生物识别元件,基于PEDOT-AuNPs复合材料和多功能石墨烯复合材料(AuNPs@MGR)制备了一种新型的电化学适配体传感器用以高灵敏检测氨苄西林。复合材料的结构通过SEM来研究,传感器的电化学性能通过CV和DPV来研究。在最佳实验条件下,该传感器具有良好的线性响应范围(0.1-200 ng/mL)和较低的检测限(0.041 ng/mL,S/N=3)。3.基于AuNPs/MWCNTs-Fe_3O_4NPs/CNS复合材料构建电化学适配体传感器检测氯霉素:以氯霉素适配体为生物识别元件,基于碳纳米球(CNS)、磁性多壁碳纳米管复合材料(MWCNTs-Fe_3O_4NPs)和AuNPs复合材料制备了一种新颖电化学适配体传感器,实现了对氯霉素的高灵敏度高选择性检测。所用材料的结构通过SEM和TEM来研究,传感器的电化学性能通过CV和DPV来研究。在最佳实验条件下,传感器具有较宽的线性范围(0.05-100 ng/mL),检测限低至0.021 ng/mL。此外,三种电化学适配体传感器均具有良好的灵敏度、稳定性、重现性和特异性,并均已成功地应用于实际样品检测。
[Abstract]:Antibiotics are widely used to treat animal and human diseases and are also used as growth-promoting factors in animal husbandry. Improper use and overuse of antibiotics can result in residues of antibiotics in ecosystems and animal-derived foods. Because of its threat to public health and ecosystem, antibiotic residues have attracted more and more attention. Therefore, it is necessary to develop an efficient and sensitive method for the detection of antibiotics. In this paper, three kinds of electrochemical aptamer sensors with excellent performance were prepared by combining advanced nanotechnology with aptamer sensor technology. The high efficiency and sensitivity of detection of penicillin, ampicillin and chloramphenicol were achieved respectively. The main contents of this paper are as follows: 1. Based on PEDOT-AuNPs / GR-Fe3O4NPs composite, electrochemical aptamer sensor was constructed for penicillin detection: penicillin aptamer was used as biometric element, magnetic graphene nanocomposite (GR-Fe3O4NPs) and poly (34-ethylenedioxythiophene gold nanoparticles) composite (PEDOT-AuNPs) were used as substrates. A novel electrochemical aptamer sensor for sensitive detection of penicillin was prepared. The structure of the composites was investigated by scanning electron microscopy (SEM). The electrochemical properties of the sensors were investigated by cyclic voltammetry (CV), electrochemical impedance (EIS) and differential pulse voltammetry (DPV). Under the optimal conditions, the sensor has a high sensitivity, the linear range is 0.1-200ng / mLand the detection limit is 0.057 ng / mL-1. Electrochemical aptamer sensor based on AuNPs / MGR/ PEDOT-AuNPs composite for ampicillin detection: ampicillin aptamer is used as biometric element. A novel electrochemical aptamer sensor based on PEDOT-AuNPs composite and multifunctional graphene composite (AuNPsMGR) was developed for the sensitive detection of ampicillin. The structure of the composite was studied by SEM, and the electrochemical properties of the sensor were studied by CV and DPV. Under the optimum experimental conditions, the sensor has a good linear response range (0.1-200ng / mL) and a lower detection limit (0.041 ng / mL-1 / N ~ (3) .3. Electrochemical aptamer sensor based on AuNPs / MWCNTs-Fe3OS / CNS composite to detect chloramphenicol: using chloramphenicol aptamer as biometric element, Based on carbon nanospheres (CNS), magnetic multiwall carbon nanotube composites (MWCNTs-Fe3O4NPs) and AuNPs composites, a novel electrochemical aptamer sensor was prepared, which can detect chloramphenicol with high sensitivity and selectivity. The structure of the material was studied by SEM and TEM, and the electrochemical performance of the sensor was studied by CV and DPV. Under the optimum experimental conditions, the sensor has a wide linear range (0.05-100 ng / mL), and the detection limit is as low as 0.021 ng / mL. In addition, the three kinds of electrochemical aptamer sensors have good sensitivity, stability, reproducibility and specificity, and have been successfully applied to the detection of practical samples.
【学位授予单位】：济南大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：TQ465;O657.1;TP212
本文编号：2131625
[Abstract]:Antibiotics are widely used to treat animal and human diseases and are also used as growth-promoting factors in animal husbandry. Improper use and overuse of antibiotics can result in residues of antibiotics in ecosystems and animal-derived foods. Because of its threat to public health and ecosystem, antibiotic residues have attracted more and more attention. Therefore, it is necessary to develop an efficient and sensitive method for the detection of antibiotics. In this paper, three kinds of electrochemical aptamer sensors with excellent performance were prepared by combining advanced nanotechnology with aptamer sensor technology. The high efficiency and sensitivity of detection of penicillin, ampicillin and chloramphenicol were achieved respectively. The main contents of this paper are as follows: 1. Based on PEDOT-AuNPs / GR-Fe3O4NPs composite, electrochemical aptamer sensor was constructed for penicillin detection: penicillin aptamer was used as biometric element, magnetic graphene nanocomposite (GR-Fe3O4NPs) and poly (34-ethylenedioxythiophene gold nanoparticles) composite (PEDOT-AuNPs) were used as substrates. A novel electrochemical aptamer sensor for sensitive detection of penicillin was prepared. The structure of the composites was investigated by scanning electron microscopy (SEM). The electrochemical properties of the sensors were investigated by cyclic voltammetry (CV), electrochemical impedance (EIS) and differential pulse voltammetry (DPV). Under the optimal conditions, the sensor has a high sensitivity, the linear range is 0.1-200ng / mLand the detection limit is 0.057 ng / mL-1. Electrochemical aptamer sensor based on AuNPs / MGR/ PEDOT-AuNPs composite for ampicillin detection: ampicillin aptamer is used as biometric element. A novel electrochemical aptamer sensor based on PEDOT-AuNPs composite and multifunctional graphene composite (AuNPsMGR) was developed for the sensitive detection of ampicillin. The structure of the composite was studied by SEM, and the electrochemical properties of the sensor were studied by CV and DPV. Under the optimum experimental conditions, the sensor has a good linear response range (0.1-200ng / mL) and a lower detection limit (0.041 ng / mL-1 / N ~ (3) .3. Electrochemical aptamer sensor based on AuNPs / MWCNTs-Fe3OS / CNS composite to detect chloramphenicol: using chloramphenicol aptamer as biometric element, Based on carbon nanospheres (CNS), magnetic multiwall carbon nanotube composites (MWCNTs-Fe3O4NPs) and AuNPs composites, a novel electrochemical aptamer sensor was prepared, which can detect chloramphenicol with high sensitivity and selectivity. The structure of the material was studied by SEM and TEM, and the electrochemical performance of the sensor was studied by CV and DPV. Under the optimum experimental conditions, the sensor has a wide linear range (0.05-100 ng / mL), and the detection limit is as low as 0.021 ng / mL. In addition, the three kinds of electrochemical aptamer sensors have good sensitivity, stability, reproducibility and specificity, and have been successfully applied to the detection of practical samples.
【学位授予单位】：济南大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：TQ465;O657.1;TP212
【参考文献】
相关期刊论文 前3条
1 王周平;张维潇;;适配体及其研究进展[J];食品与生物技术学报;2013年09期
2 周玲;王明华;王剑平;叶尊忠;;传感器表面的适配体固定方法及其在生物传感器中的研究进展[J];分析化学;2011年03期
3 张怀;张云怀;李静;肖鹏;乔雷;李泽全;樊小燕;;DNA共价修饰单壁碳纳米管电极的制备及与VB_6相互作用的研究[J];分析测试学报;2008年08期
相关博士学位论文 前1条
1 李赫楠;功能纳米材料的制备及生物传感应用[D];东南大学;2015年
，本文编号：2131625
本文链接：https://www.wllwen.com/kejilunwen/huaxue/2131625.html
教材专著
