应用高效液相色谱-二极管阵列检测技术测定阿卡波糖片含量的研究
发布时间：2018-07-21 20:58
【摘要】：目的应用高效液相色谱-二极管阵列检测(HPLC-DAD)技术,建立阿卡波糖片中阿卡波糖的含量测定方法。方法采用Alltima C_(18)色谱柱(4.6 mm×250 mm,5μm),以乙腈~(-1)0 mmol·L~(-1)磷酸二氢铵溶液(含0.04%辛烷磺酸钠,磷酸调至pH 3.3)(15∶85)为流动相,流速0.8 m L·min~(~(-1)),检测波长200 nm,柱温30℃。结果在本方法中,阿卡波糖的线性关系良好,线性相关系数(r)为1.000 0;专属性强,阿卡波糖和药品中其他成分以及强制降解产物之间均有良好的分离度;精密度和稳定性良好,RSD分别为0.088%和0.22%;回收率良好,3个加样浓度的平均回收率(n=9)为98.49%,RSD为0.24%。对4批阿卡波糖片进行测定,含量分别为每片51.69、51.75、51.72、51.62 mg。结论该方法准确、简单、快速,可作为阿卡波糖片中阿卡波糖的含量测定方法。
[Abstract]:Objective to establish a method for the determination of acarbose in acarbose tablets by high performance liquid chromatography-diode array detection (HPLC-DAD). Methods Alltima C _ (18) column (4.6 mm 脳 250mm ~ (-1) 渭 m),) was used as mobile phase with acetonitrile ~ (-1) 0 mmol L ~ (-1) ammonium dihydrogen phosphate solution (0.04% sodium octanesulfonate, adjusted phosphoric acid to pH 3.3) (15:85), flow rate was 0.8 mL 路min ~ (-1), detection wavelength was 200nm, column temperature was 30 鈩，
本文编号：2136834
[Abstract]:Objective to establish a method for the determination of acarbose in acarbose tablets by high performance liquid chromatography-diode array detection (HPLC-DAD). Methods Alltima C _ (18) column (4.6 mm 脳 250mm ~ (-1) 渭 m),) was used as mobile phase with acetonitrile ~ (-1) 0 mmol L ~ (-1) ammonium dihydrogen phosphate solution (0.04% sodium octanesulfonate, adjusted phosphoric acid to pH 3.3) (15:85), flow rate was 0.8 mL 路min ~ (-1), detection wavelength was 200nm, column temperature was 30 鈩，
本文编号：2136834
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