纳米铜修饰的非酶葡萄糖传感器性能研究
发布时间：2018-07-25 08:18
【摘要】：糖尿病严重威胁了人类的健康,过去的几十年里,酶葡萄糖传感器为人类带来了福音。目前酶传感器仍然存在酶易缺失、重复稳定性和贮存稳定性差以及易受外界噪音干扰等缺点。非酶传感器可以部分克服这些缺点,得到人们越来越多的重视。本文以ITO为基底,以电化学法将ITO电极用纳米铜和纳米镍修饰,用于单糖(葡萄糖和果糖)和二糖(乳糖、麦芽糖和蔗糖)的检测。研究表明,Cu/Ni/ITO电极对单糖和二糖都有较好的灵敏度,但是检测单糖的灵敏度要高于二糖。其检测原理可以总结为:在电极的催化氧化下醛基转换成羧酸,导致电极表面电导率的变化和氧化峰出现。尽管果糖不是葡萄糖,但它是葡萄糖的同分异构体,在碱性环境下可以异构化为葡萄糖从而被检测。二糖也能被检测,这是因为部分二糖在碱性环境下也能发生异构化反应产生醛基,进而被检测。进一步的实验表明,纳米颗粒的尺寸和形态影响了其检测性能。用磁控溅射法在ITO基底生长了Au-Pd复合膜作电极,用电化学方法在电极上生长纳米铜就可以得到的Cu/Au-Pd/ITO电极,其葡萄糖的检测效果明显好于Cu/ITO电极和Au-Pd/ITO电极,检测的灵敏度和响应电流强度明显提高,且响应电流与葡萄糖浓度之间呈明显的线性关系。形貌分析表明,较之其它电极,Cu/Au-Pd/ITO电极纳米颗粒尺寸更小,单位面积分布致密均匀,对葡萄糖检测性能更好。循环伏安曲线表明,Cu/Au-Pd/ITO电极具有更接近0 V的氧化还原电位,这说明纳米铜和纳米Au-Pd结合形成的裸露界面增强了其检测葡萄糖的催化活性。这对于开发低驱动电压的非酶葡萄糖传感器具有重要意义。
[Abstract]:Diabetes is a serious threat to human health. Over the past few decades, enzyme-glucose sensors have brought good news. At present, enzyme sensors still have some disadvantages, such as easy absence of enzyme, poor stability of repeat and storage, and easy to be disturbed by external noise. Non-enzyme sensors can partly overcome these shortcomings, and people pay more and more attention to them. In this paper, ITO electrode was modified with nano-copper and nano-nickel by electrochemical method on ITO substrate, and was used for the detection of monosaccharides (glucose and fructose) and disaccharides (lactose, maltose and sucrose). The results show that Cu- / Ni- / ITO electrode has better sensitivity to both monosaccharides and disaccharides, but the sensitivity of monosaccharides is higher than that of monosaccharides. The detection principle can be summarized as follows: the conversion of aldehyde group to carboxylic acid under the catalytic oxidation of the electrode leads to the change of the surface conductivity of the electrode and the appearance of the oxidation peak. Although fructose is not glucose, it is the isomer of glucose, which can be isomerized to glucose in alkaline environment and detected. Disaccharides can also be detected because some of them can also be isomerized in alkaline environments to produce aldehyde groups, which are then detected. Further experiments show that the size and morphology of nanoparticles affect their detection performance. The Au-Pd composite film was grown on ITO substrate by magnetron sputtering, and the Cu/Au-Pd/ITO electrode was obtained by electrochemical growth of nano-copper on the electrode. The detection effect of glucose was better than that of Cu/ITO electrode and Au-Pd/ITO electrode. The sensitivity and the response current intensity were improved obviously, and the linear relationship between the response current and glucose concentration was obvious. The morphology analysis shows that compared with other electrode Cu-Au-PD / ITO electrode, the size of nanoparticles is smaller, the distribution of unit area is compact and uniform, and the performance of glucose detection is better. The cyclic voltammetry curves show that the Cu / au / PD / ITO electrode has a redox potential closer to 0 V, which indicates that the exposed interface formed by the combination of nano-copper and Au-Pd enhances its catalytic activity for glucose detection. This is of great significance for the development of non-enzymatic glucose sensors with low driving voltage.
【学位授予单位】：吉林大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：O657.1
[Abstract]:Diabetes is a serious threat to human health. Over the past few decades, enzyme-glucose sensors have brought good news. At present, enzyme sensors still have some disadvantages, such as easy absence of enzyme, poor stability of repeat and storage, and easy to be disturbed by external noise. Non-enzyme sensors can partly overcome these shortcomings, and people pay more and more attention to them. In this paper, ITO electrode was modified with nano-copper and nano-nickel by electrochemical method on ITO substrate, and was used for the detection of monosaccharides (glucose and fructose) and disaccharides (lactose, maltose and sucrose). The results show that Cu- / Ni- / ITO electrode has better sensitivity to both monosaccharides and disaccharides, but the sensitivity of monosaccharides is higher than that of monosaccharides. The detection principle can be summarized as follows: the conversion of aldehyde group to carboxylic acid under the catalytic oxidation of the electrode leads to the change of the surface conductivity of the electrode and the appearance of the oxidation peak. Although fructose is not glucose, it is the isomer of glucose, which can be isomerized to glucose in alkaline environment and detected. Disaccharides can also be detected because some of them can also be isomerized in alkaline environments to produce aldehyde groups, which are then detected. Further experiments show that the size and morphology of nanoparticles affect their detection performance. The Au-Pd composite film was grown on ITO substrate by magnetron sputtering, and the Cu/Au-Pd/ITO electrode was obtained by electrochemical growth of nano-copper on the electrode. The detection effect of glucose was better than that of Cu/ITO electrode and Au-Pd/ITO electrode. The sensitivity and the response current intensity were improved obviously, and the linear relationship between the response current and glucose concentration was obvious. The morphology analysis shows that compared with other electrode Cu-Au-PD / ITO electrode, the size of nanoparticles is smaller, the distribution of unit area is compact and uniform, and the performance of glucose detection is better. The cyclic voltammetry curves show that the Cu / au / PD / ITO electrode has a redox potential closer to 0 V, which indicates that the exposed interface formed by the combination of nano-copper and Au-Pd enhances its catalytic activity for glucose detection. This is of great significance for the development of non-enzymatic glucose sensors with low driving voltage.
【学位授予单位】：吉林大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：O657.1
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