UPC~2-MS法快速检测蜂蜜中的果糖、葡萄糖、蔗糖和麦芽糖
发布时间：2018-07-25 20:11
【摘要】：建立了蜂蜜中果糖、葡萄糖、蔗糖和麦芽糖的超高效合相色谱-质谱(UPC2-MS)快速检测的方法。样品先用少量的水溶解,再用正己烷/异丙醇(1:1,V/V)溶解,经ACQUITY UPC2BEH(100 mm×3.0 mm,1.7μm)色谱柱分离,以超临界CO2-甲醇(含0.1%NH3·H2O,V/V)为流动相进行梯度洗脱,流速为1.8 m L/min,通过质谱检测器在负离子电喷雾模式下对目标化合物进行分析,外标法定量。检测结果表明:果糖、葡萄糖、蔗糖和麦芽糖在2~150 mg/L范围线性关系良好,R20.9990,定量限(LOQ,S/N≥10)为0.22~0.42 mg/L,高、中、低3个浓度水平的加标平均回收率在90.0%~107.5%范围,相对标准偏差(RSD,n=6)均小于4.0%。该方法简单快速,分离效果好,为蜂蜜中果糖、葡萄糖、蔗糖和麦芽糖的分离测定提供了新的色谱技术平台。
[Abstract]:A rapid method for the determination of fructose, glucose, sucrose and maltose in honey by superhigh performance phase chromatography-mass spectrometry (UPC2-MS) was established. The sample was dissolved in a small amount of water, then dissolved with n-hexane / isopropanol (1: 1 V / V). The sample was separated by ACQUITY UPC2BEH (100mm 脳 3.0mm ~ (1.7) 渭 m) column. The supercritical CO _ 2-methanol (containing 0.1%NH3 H _ 2O _ 2O _ V) was used as the mobile phase for gradient elution. At a flow rate of 1.8 mL / min, the target compounds were analyzed by mass spectrometer in anion electrospray mode and quantified by external standard method. The results showed that the linear range of fructose, glucose, sucrose and maltose was 20. 9990 in the range of 2 ~ 150 mg/L. The quantitative limit (LOQN / N 鈮，
本文编号：2144945
[Abstract]:A rapid method for the determination of fructose, glucose, sucrose and maltose in honey by superhigh performance phase chromatography-mass spectrometry (UPC2-MS) was established. The sample was dissolved in a small amount of water, then dissolved with n-hexane / isopropanol (1: 1 V / V). The sample was separated by ACQUITY UPC2BEH (100mm 脳 3.0mm ~ (1.7) 渭 m) column. The supercritical CO _ 2-methanol (containing 0.1%NH3 H _ 2O _ 2O _ V) was used as the mobile phase for gradient elution. At a flow rate of 1.8 mL / min, the target compounds were analyzed by mass spectrometer in anion electrospray mode and quantified by external standard method. The results showed that the linear range of fructose, glucose, sucrose and maltose was 20. 9990 in the range of 2 ~ 150 mg/L. The quantitative limit (LOQN / N 鈮，
本文编号：2144945
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