在线二维柱切换-超高效液相色谱法同时测定明目地黄丸中莫诺苷、马钱苷、芍药苷、丹皮酚的含量

发布时间：2018-07-26 13:19

【摘要】：建立了二维柱切换-超高效液相色谱法同时测定明目地黄丸中莫诺苷、马钱苷、芍药苷、丹皮酚含量的方法。一维色谱柱为Thermo Accucore XL C_(18)(250 mm×2.1 mm,4μm),二维色谱柱为DIONEX Acclaim phenyl-1(150 mm×4.6 mm,3μm),一维分析流动相为乙腈-水,梯度洗脱,二维分析流动相为乙腈-水,等度洗脱,14.5 min进行阀切换;检测波长:0~14.5 min为240 nm,14.5~30 min为275 nm,流速:0.5 m L/min,柱温:30℃。30 min即可完成明目地黄丸中莫诺苷、马钱苷、芍药苷、丹皮酚的含量测定,并有效地将莫诺苷同分异构体分离。莫诺苷、马钱苷、芍药苷、丹皮酚的线性范围分别为7.6~377 mg/L,9.2~459 mg/L,8.4~419 mg/L和8.2~409 mg/L,相关系数为0.9999,加样回收率为98.3%~100.2%。本方法快捷高效,可对控制明目地黄丸质量提供参考。
[Abstract]:A method for simultaneous determination of monoside, marganin, paeoniflorin and paeonol in Mingmu Rehmannia pills by two dimensional column switching and ultra-high performance liquid chromatography was established. One dimensional column was Thermo Accucore XL C _ (18) (250mm 脳 2.1mm ~ 4 渭 m),). Two-dimensional column was DIONEX Acclaim phenyl-1 (150mm 脳 4.6mm ~ 3 渭 m),). The mobile phase was acetonitrile-water, gradient elution was gradient elution. The mobile phase was acetonitrile-water, and the isometric elution was 14.5 min for valve switching. The detection wavelength of 14.5 min was 240nmL 14.5 min, the flow rate was 275nm / min, the column temperature was 30 鈩，

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