新型共轭聚电解质—贵金属纳米复合体系的生化传感研究

发布时间：2018-07-29 17:18

【摘要】：金、银纳米粒子具有独特的光学、电学和化学性质,在材料、信息及生命科学等领域显示出广阔的应用前景。因此,金、银纳米粒子及其复合材料的制备与应用已经成为了当今科学研究的热点领域之一。本文合成了水溶性共轭聚电解质(P1、PPESO3、P2)稳定的贵金属纳米复合材料,用于构建三聚氰胺、生物硫醇及依替米星灵敏检测的传感体系。具体内容包括以下三个部分:(1)设计合成了一种新型纳米比率探针P1-AgNPs,实现了三聚氰胺高灵敏、高选择性的荧光及比色检测。含吡啶基团的P1不仅是优良的双发射荧光探针,还起到稳定银纳米颗粒的作用。随着三聚氰胺的加入,由于三聚氰胺的氨基与银纳米优异的结合能力,形成了三聚氰胺覆盖的P1-AgNPs,使得P1在386 nm处的荧光部分恢复,与此同时由于三聚氰胺诱导的团聚,导致P1在488 nm处的荧光进一步猝灭。因而可以利用比率荧光检测三聚氰胺。另外,三聚氰胺诱导P1-AgNPs的团聚现象可以用于比率比色法检测三聚氰胺。荧光法和比色法检测三聚氰胺的检出限低至0.1和0.45 nM。(2)以荧光共轭聚电解质PPESO3为稳定剂、以硼氢化钠为还原剂合成了平均粒径约5 nm的金纳米复合材料(PPESO3-AuNPs)。PPESO3的荧光可以被金纳米粒子猝灭,体系中加入含巯基的生物分子(Cys、Hcy)后,由于巯基更易与金纳米粒子作用,从而使得PPESO3的荧光恢复,这为生物硫醇的灵敏检测提供了一种切实可行的方法,还可用于HeLa细胞中生物硫醇的检测。(3)设计合成了荧光共轭聚电解质P2-AgNPs复合体系用于依替米星的荧光及比色检测。在最优条件下,荧光和比色检测依替米星的检测限分别为8 nM和100 nM。
[Abstract]:Gold and silver nanoparticles have unique optical, electrical and chemical properties, and have shown wide application prospects in materials, information and life sciences. Therefore, the preparation and application of gold, silver nanoparticles and their composites have become one of the hot fields of scientific research. In this paper, water soluble conjugated polyelectrolyte (P1PPESO3 / P2) stabilized noble metal nanocomposites were synthesized and used to construct sensitive sensing systems for melamine, bio-mercaptan and etimicin. The specific contents include the following three parts: (1) A novel nano-ratio probe P1-AgNPs was designed and synthesized to achieve a highly sensitive and selective fluorescence and colorimetric detection of melamine. P1 containing pyridine group is not only a good double emission fluorescence probe, but also plays a role in stabilizing silver nanoparticles. With the addition of melamine, due to the excellent binding ability of melamine amino groups to silver nanoparticles, melamine-coated P1-AgNPs were formed, which partially recovered P1 fluorescence at 386 nm, and at the same time due to melamine induced agglomeration. The fluorescence of P1 is further quenched at 488 nm. Therefore, melamine can be detected by ratio fluorescence. In addition, the agglomeration of P1-AgNPs induced by melamine can be used for the determination of melamine by ratio colorimetry. The detection limits of melamine by fluorescence and colorimetry were as low as 0.1 and 0.45 nm. (2) fluorescent conjugated polyelectrolyte PPESO3 was used as stabilizer. The fluorescence of gold nanocomposites (PPESO3-AuNPs. PPESO3) with average diameter of about 5 nm was synthesized with sodium borohydride as reducing agent. The fluorescence of gold nanoparticles could be quenched by gold nanoparticles. Thus, the fluorescence of PPESO3 is restored, which provides a feasible method for the sensitive detection of biological mercaptan. It can also be used for the detection of biological mercaptan in HeLa cells. (3) the fluorescent conjugated polyelectrolyte P2-AgNPs composite system was designed and synthesized for the fluorescence and colorimetric detection of etimicin. Under the optimal conditions, the detection limits of Etimicin for fluorescence and colorimetric detection are 8 nm and 100 nm, respectively.
【学位授予单位】：湖南师范大学
【学位级别】：硕士
【学位授予年份】：2016
【分类号】：O657.3

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