甲烷氧化菌素功能化金纳米层层自组装修饰电极上过氧化氢的催化还原

发布时间：2018-08-02 15:07

【摘要】：借助巯基试剂,在纳米金颗粒表面修饰生物活性物质Mb,制备保持有Mb生物活性的功能化金纳米巯基乙胺-Au NPs-Mb.采用UV-Vis、FTIR光谱和投射电镜表征其结构,该纳米颗粒分布均匀且粒径均一,并显著改善了金纳米颗粒团聚现象.以Mb功能化金纳米为基元,采用单层自组装及层层自组装方式将其修饰到裸金电极表面.各Mb或Mb-Cu电极的电化学测试并未借助电子传递媒介.配位Cu~(2+)后,修饰有Mb的单层及层层自组装修饰的催化还原能力均显著提升.其中Cu~(2+)配位的{巯基乙胺-Au NPs-Mb}3/Au修饰电极作为一种新型H2O2生物传感器,响应时间大约为2 s,米氏常数KappM为0.787 mmol/L,表现出了较强的还原H2O2的催化活性,且稳定性较好.
[Abstract]:The functional gold nano-mercaptoethylamine -Au NPs-Mb was prepared by modifying the bioactive substance Mb on the surface of gold nanoparticles with sulfhydryl reagent. The structure of the nanoparticles was characterized by UV-Vis-IR spectra and projection electron microscopy. The nanoparticles were uniformly distributed and uniform, and the aggregation of gold nanoparticles was improved significantly. The Mb functionalized gold nanoparticles were modified to the bare gold electrode by monolayer self-assembly and layer-by-layer self-assembly. The electrochemical measurements of each Mb or Mb-Cu electrode did not rely on electron transfer media. The catalytic reduction ability of Mb modified monolayer and layer by layer self-assembly modification was significantly improved after the coordination of Cu2. As a new type of H2O2 biosensor, the Cu ~ (2) coordinated {mercaptoethylamine -Au NPs-Mb} 3/Au modified electrode is a new type of H2O2 biosensor with a response time of about 2 s and a KappM constant of 0.787 mmol / L, showing strong catalytic activity of reducing H2O2 and good stability.
【作者单位】：哈尔滨商业大学食品科学与工程重点实验室;中国科学院兰州化学物理研究所羰基合成与选择氧化国家重点实验室;
【基金】：国家自然科学基金(21573055;21073050) 哈尔滨商业大学研究团队支撑项目(2016TD006) 国家重点实验室项目合作项目(cre-2015-c-104)~~
【分类号】：O646

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