快速检测镉离子的免疫层析分析法的建立及应用

发布时间：2018-08-02 18:39

【摘要】：在制备出镉离子单克隆抗体的基础上,首先运用柠檬酸钠还原法合成了胶体金,然后用胶体金标记抗体,得到检测探针;优化实验条件后,组装试纸条,样品中的镉与检测线上面的抗原竞争有限的金标抗体,通过观察检测线的颜色,确定检出限;最后检测方法的特异性和灵敏度,建立了一种快速检测水样中镉离子的竞争免疫层析试纸条方法。结果:方法的检出限为50ng·mL-1,交叉反应结果表明,该抗体除与汞离子有交叉反应外,与其他金属离子(Zn~(2+)、Cr~(3+)、Pb~(2+)、Cu~(2+)、Ni~(2+)、Al~(3+)、Co~(2+)、Fe~(3+)、Mg~(2+)、Ca~(2+)和Ba~(2+))均无交叉反应。湖水样品中镉的加标实验结果显示与传统的酶联免疫分析方法有很好的相关性。建立的胶体金免疫层析试纸条方法可以特异、快速地检测水样中的重金属镉离子,在10min左右可以得到结果,适合用于现场检测。
[Abstract]:On the basis of the preparation of monoclonal antibody to cadmium ion, colloidal gold was synthesized by sodium citrate reduction method, then the detection probe was obtained by labeling the antibody with colloidal gold. Cadmium in the sample competes with the gold labeled antibody which is limited by the antigen above the detection line. The detection limit is determined by observing the color of the detection line. Finally, the specificity and sensitivity of the detection method are determined. A competitive immunochromatographic strip method for the rapid detection of cadmium ions in water samples was established. Results: the detection limit of the method was 50ng mL-1. The results of cross reaction showed that the antibody did not cross react with other metal ions (Zn ~ (2) C ~ (3) Cr ~ (3) Pb ~ (2) Cu ~ (2) C ~ (2) O _ (2) Al ~ (3) Co ~ (2) Fe ~ (3) Fe ~ (2) Ca ~ (2) and Ba ~ (2) except Hg ~ (2). The experimental results of cadmium addition in lake water samples showed that there was a good correlation with the traditional enzyme linked immunosorbent assay (Elisa). The method of colloidal gold immunochromatographic strip can be used to detect cadmium ions in water samples specifically and quickly. The results can be obtained about 10min, which is suitable for field detection.
【作者单位】：山西大同大学化学与环境工程学院;山西大学化学化工学院;山西大同大学有机化学研究所;
【基金】：国家自然科学基金项目(21375083)资助山西省大同市科技攻关项目(2015025)资助山西大同大学博士科研启动项目(2012-B-08)资助
【分类号】：O658.1;X832

【相似文献】