UPLC法测定香蕉、皇帝蕉皮及其果肉中羽扇豆酮含量

发布时间：2018-08-09 19:20

【摘要】：目的:建立超高效液相色谱法分离和测定不同收集期的香蕉和皇帝蕉皮及其果肉中羽扇豆酮含量的方法,并优化提取条件。方法:采用甲醇回流提取法对2种蕉皮及其果肉的羽扇豆酮进行提取分离,减压浓缩,选用Waters ACQUITY UPLC BEH C18色谱柱(2.1 mm×50 mm,1.7μm),流动相为乙腈-水(85∶15,V/V)溶液,流速0.3 mL/min,柱温30℃,检测波长206 nm。结果:香蕉和皇帝蕉果肉中均未检测出羽扇豆酮,但2种蕉皮中有较高含量的羽扇豆酮,并且在16.70~59.96 mg/100 mL质量浓度范围内有良好线性关系(r=0.999 6),羽扇豆酮平均回收率为99.00%,相对标准偏差为3.0%。结论:该法回收率高、重复性好,且具有专属性强、准确性高和简便快捷等特点,适用于香蕉、皇帝蕉皮中羽扇豆酮的含量测定。
[Abstract]:Objective: to establish a high performance liquid chromatography method for the separation and determination of Lupanone in banana, banana peel and pulp of banana and emperor in different collecting periods, and to optimize the extraction conditions. Methods: two kinds of plantain peel and pulp were extracted and concentrated by methanol reflux extraction method. Waters ACQUITY UPLC BEH C18 column (2.1 mm 脳 50 mm ~ (1.7) 渭 m),) was used as mobile phase with acetonitrile-water (85: 15 V / V) solution, flow rate was 0.3 mL / min, column temperature was 30 鈩，

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