两性聚丙烯酰胺冰胶对蛋白质分子印迹的广泛适用性

发布时间：2018-08-13 16:14

【摘要】：选择溶菌酶、胃蛋白酶、卵清蛋白、牛血红蛋白和γ-球蛋白为模板,考察两性聚丙烯酰胺冰胶的分子印迹功能。预聚溶液中除了丙烯酰胺、甲叉双丙烯酰胺外,还含有作为功能单体的丙烯酸和烯丙基胺,因此所得聚合物中既有酸性基团,也有碱性基团,有助于在分子水平上识别同样是两性物质的蛋白质。上述蛋白质的分子量、等电点差异显著,且涵盖了很宽的范围。利用同一单体溶液即可合成对所有蛋白质均有印迹效果的聚合物,其分子量和等电点无影响。模板分子在毛细管印迹柱中的保留时间大于非印迹柱中的保留时间。溶菌酶的印迹因子高达7.0,γ-球蛋白最小(2.0),其它蛋白质介于二者之间。本研究结果表明,两性聚丙烯酰胺冰胶是印迹各种蛋白质的理想材料,在蛋白质的识别、纯化和去除方面具有极大的潜力。
[Abstract]:Lysozyme, pepsin, ovalbumin, bovine hemoglobin and 纬 -globulin were selected as templates to investigate the molecular imprinting function of amphoteric polyacrylamide ice gel. In addition to acrylamide and methylene bisacrylamide, the prepolymer solution also contains acrylic acid and allylamine as functional monomers. It helps to recognize proteins at the molecular level that are also hermaphroditic substances. The molecular weight and isoelectric point of the above proteins vary significantly and cover a wide range. The polymer with imprinting effect on all proteins can be synthesized by using the same monomer solution, and its molecular weight and isoelectric point have no effect. The retention time of template in capillary imprinted column is longer than that in non-imprinted column. The imprinting factor of lysozyme was as high as 7.0, 纬 -globulin was the smallest (2.0), and the other proteins were between them. The results show that Amphoteric polyacrylamide ice gel is an ideal material for imprinting various proteins and has great potential in protein recognition, purification and removal.
【作者单位】：扬州大学化学化工学院;
【基金】：国家自然科学基金(No.21375115) 江苏高校优势学科建设工程资助项目~~
【分类号】：O631.3

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