基于荧光银纳米簇的多功能传感平台对药物及目标DNA的检测

发布时间：2018-08-14 13:01

【摘要】：金属纳米簇是近年来发展起来的一类粒径小于或接近于费子波尔半径的半导体纳米颗粒,因为其独特的光、电学性质以及良好的生物相容性、水溶性、低毒性等优点,广泛应用于化学传感、生物标记、疾病检测等分析检测领域。本文以聚乙烯亚胺为模板的银纳米簇为研究对象,构建了检测喹诺酮类抗生素、维生素B12、目标DNA和三磷酸腺苷(adenosine triphosphate,ATP)的新方法。主要研究内容如下:(1)以不同分子量(Mw=600、1300、2000、10000、25000、70000和750000)的聚乙烯亚胺(polyethyleneimine,PEI)为模板合成的银纳米簇(Ag nanoclusters,Ag NCs)为荧光探针,当向Ag NCs中添加Cu2+,随着Cu2+浓度的增大使得Ag NCs的荧光强度逐渐猝灭,通过向其系统中加入喹诺酮类药物(包括萘啶酸、西诺沙星、环丙沙星、氧氟沙星、莫西沙星)使得Ag NCs的荧光逐渐恢复。同时,这一传感系统(Ag NCs-Cu2+)对上述喹诺酮类药物的检测显示出了良好的线性范围和较低的检出限。此外,该传感器系统已成功用于人血清和尿中喹诺酮类药物的测定。(2)以不同分子量(Mw=600、10000和70000)以及不同封端基团(乙二胺和乙氧基)的PEI为模板合成的Ag NCs作为一种新型荧光探针,随着维生素B12浓度的增大,由于内滤效应,其可以猝灭Ag NCs的荧光。同时,该方法选择性好、灵敏度高,已被成功用于注射剂和片剂样品中维生素B12的测定。(3)血红素适配体与血红素可形成复合物,该复合物与Ag NCs之间发生光诱导电子转移(photoinduced electron transfer,PET)猝灭Ag NCs的荧光。以此为实验机理,以分子量为600的PEI包裹的Ag NCs作为荧光探针,本章构建了具有广泛适用性的传感平台,通过改变DNA序列,建立了检测目标DNA和ATP的分析方法。以目标DNA/ATP和血红素作为输入信号,以Ag NCs的荧光变化作为输出信号,构建荧光“AND”逻辑门。
[Abstract]:Metal nanoclusters are a kind of semiconductor nanoparticles whose particle size is less than or close to the Fertilizer radius developed in recent years, because of its unique light, electrical properties and good biocompatibility, water solubility, low toxicity and so on. Widely used in chemical sensing, biomarker, disease detection and other analytical field. In this paper, a new method for the detection of quinolones, vitamin B12, target DNA and adenosine triphosphate ATP was constructed with polyimide as the template of silver nanoclusters. The main research contents are as follows: (1) Ag nanoclusters (Ag nanoclusters Ag NCs) with different molecular weights (MwC600,000,000,000,000,000,000,000,000,000,000,000,000,000,000,000,000,000,000,000,000,000,000,000,000,000,000,000,000,000,000,000,000,000,000,000,000,000,000,000,000,000,000,000,000,000,000,000,000,000,000,000,000,000,000,000,000,000,000,000,000@@ The fluorescence of Ag NCs was gradually restored by adding quinolones (including naphthalic acid, ciprofloxacin, ofloxacin, moxifloxacin) into the system. At the same time, this sensing system (Ag NCs-Cu2) showed good linear range and low detection limit for quinolones. In addition, the sensor system has been successfully used for the determination of quinolones in human serum and urine. (2) Ag NCs with different molecular weights (MwC600,10000 and 70000) and PEI with different entrapment groups (ethylenediamine and ethoxy) as a new fluorescent probe, has been successfully used for the determination of quinolones in human serum and urine. With the increase of vitamin B12 concentration, the fluorescence of Ag NCs can be quenched due to the internal filtration effect. At the same time, the method has good selectivity and high sensitivity. It has been successfully used for the determination of vitamin B12 in injection and tablet samples. (3) heme aptamer can form complex with heme. The fluorescence of Ag NCs was quenched by photo-induced electron transfer (photoinduced electron transfer PET between the complex and Ag NCs. Based on the experimental mechanism, using Ag NCs encapsulated by PEI with molecular weight 600 as fluorescence probe, a widely applicable sensing platform is constructed in this chapter. By changing the DNA sequence, an analytical method for detecting target DNA and ATP is established. The target DNA/ATP and heme were used as input signals, and the fluorescence changes of Ag NCs were used as output signals to construct the fluorescent "AND" logic gate.
【学位授予单位】：曲阜师范大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R927;O657.3;TQ460.72

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