超高效液相色谱-高分辨质谱法同时测定类叶牡丹中9种三萜皂苷类成分

发布时间：2018-08-14 20:07

【摘要】：目的建立超高效液相色谱-高分辨质谱法(UPLC-FT/MS)同时测定类叶牡丹Cauorlhyllum robustum中9种三萜皂苷类成分的方法。方法采用正离子模式;色谱柱:ACE Excel 3 C_(18)(100 mm×2.1 mm,3.0μm);保护柱:ACE Excel 3C_(18)(12.5 mm×4.6 mm,3.0μm);流动相为0.1%甲酸水(A)-乙腈(B)溶液,梯度洗脱,体积流量0.40 m L/min,柱温35℃。结果定量分析的类叶牡丹中三萜皂苷类成分在考察的浓度范围内呈良好的线性关系(r0.996);加样回收率和RSD分别在98.49%~107.03%和1.00%~3.33%。结论本研究建立的同时测定类叶牡丹中9种三萜皂苷类成分的UPLC-FT/MS定量分析方法灵敏度高、分辨率高、质量稳定并且简便、准确,可为综合评价类叶牡丹的质量提供参考。
[Abstract]:Objective to establish a high performance liquid chromatography-high resolution mass spectrometry (UPLC-FT/MS) method for the simultaneous determination of 9 triterpenoid saponins in Cauorlhyllum robustum. Methods Excel 3C18 (100mm 脳 2.1mm) Excel 3C18 (12.5mm 脳 4.6mm) 3.0 渭 m); mobile phase was gradient eluted with 0.1% formic acid water (A) acetonitrile (B) solution, volume flow rate was 0.40 mL / min, column temperature was 35 鈩，

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