中药成分香草酸和咖啡酸的荧光分析方法研究

发布时间：2018-08-16 14:15

【摘要】：本文研究了中药活性成分香草酸、咖啡酸、咖啡酸乙酯的荧光性质,探讨了中药材胡黄连、中成药肥儿丸、黄连羊肝丸中的香草酸以及中药材蒲公英中的咖啡酸的定量分析方法。实验中,用三维荧光技术获取标准溶液和样品溶液全面的荧光信息,通过比较三维荧光图谱的一致性对样品中可能存在的干扰做出初步判断,然后采用荧光分光光度法和基于交替三线性分解的二阶校正技术处理光谱数据,以选择合适的分析方法。论文工作主要包括以下三部分:1、研究了香草酸的荧光性质。香草酸内源荧光很弱,但在香草酸水溶液中加入AlCl3可使荧光明显增强,此荧光在弱酸性条件下稳定,最大激发波长λex为260 nm,最大发射波长λem为344 nm。探究了实验条件对香草酸敏化荧光的影响。以L-色氨酸为参比,测得香草酸-Al(Ⅲ)络合物的荧光量子产率为0.21,属于强荧光物质。2、基于香草酸的荧光性质,用荧光分光光度法测定了中药材胡黄连、中成药肥儿丸、黄连羊肝丸香草酸的含量,测定结果为:胡黄连含香草酸0.434%、肥儿丸含香草酸0.106%,黄连羊肝丸含香草酸0.0344%。用三维荧光二阶校正法对上述样品进行测定,结果分别为:0.373%、0.101%和0.0303%。通过对比可见,两种方法对肥儿丸的测定结果基本一致,但对胡黄连和黄连羊肝丸的测定不同,表明三维荧光二阶校正法对共存组分的干扰有一定的分辨能力。3、研究了咖啡酸和咖啡酸乙酯的荧光性质,探究了光照对咖啡酸荧光强度的影响,发现光照能使咖啡酸的荧光增强。用荧光分光光度法对蒲公英中的咖啡酸进行定量分析,测定结果为0.0232%,用三维荧光二阶校正法测定结果为0.0240%,两者基本一致。本节还探讨了用三维荧光二阶校正法对咖啡酸和咖啡酸乙酯混合溶液中两种组分的含量分别进行测定的可行性,得到了有意义的结果。
[Abstract]:The fluorescence properties of vanillic acid and ethyl caffeic acid were studied in this paper. Quantitative analysis of vanillic acid in Huanglian Yanggan pills and caffeic acid in dandelion. In the experiment, the comprehensive fluorescence information of standard solution and sample solution was obtained by using three-dimensional fluorescence technique, and the possible interference in the sample was preliminarily judged by comparing the consistency of the three-dimensional fluorescence spectrum. Then the fluorescence spectrophotometry and the second-order correction technique based on alternating trilinear decomposition were used to process the spectral data in order to select an appropriate analytical method. In this paper, the fluorescence properties of vanillic acid were studied in the following three parts: 1. The endogenous fluorescence of vanillic acid is very weak, but the fluorescence of vanillic acid aqueous solution can be enhanced by adding AlCl3. The fluorescence is stable under weak acid condition. The maximum excitation wavelength 位 ex is 260 nm and the maximum emission wavelength 位 em is 344 nm. The effects of experimental conditions on vanillic acid-sensitized fluorescence were investigated. The fluorescence quantum yield of vanillic acid-Al (鈪，

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