表面印迹技术对环境激素双酚A的高效吸附分离与检测

发布时间：2018-08-20 17:05

【摘要】：双酚A作为一种环境雌激素,对人类以及其他生命体具有很大的危害。当双酚A进入生命机体后,会干扰正常的内分泌物的合成,造成机体内各大系统的紊乱,从而产生各种疾病危害健康,甚至影响人类繁衍。因此,分离检测双酚A有着重要意义。近年来,管内固相微萃取用于分离检测痕量双酚A,由于其在实现自动化、低成本、环境友好等方面的优势得到了蓬勃发展。然而,这些萃取介质对双酚A没有特异选择性,容易受到基质或共存物干扰,影响分析准确性和灵敏度。表面分子印迹技术制备的聚合物对目标分子具有一定的选择性,在双酚A的分离检测中也得到了广泛的应用。然而,表面分子印迹聚合物也存在一定的非特异性吸附。适配体作为一种高特异性识别材料在双酚A的特异性检测方面,已经得到广泛运用。本论文结合管内固相微萃取、表面分子印迹技术以及适配体的高特异性识别能力,对双酚A的高效分离与检测进行了研究,为双酚A的分离与检测提供了一定的参考价值。具体研究内容如下:1、通过适配体亲和双酚A定轨表面印迹技术制备了特异性识别双酚A的分子印迹聚合物。对获得的适配体-表面分子印迹聚合物微球和普通的表面分子印迹聚合物微球的吸附性能和选择性能进行了测试比较,实验发现适配体-表面分子印迹聚合物微球,这种印迹方式提高了模板利用率,可以形成更密集的氢键的位点。适配体表面分子印迹的吸附能力是普通表面分子印迹的两倍,且印迹孔穴中的适配体能对目标分子具有较高的选择性。这表明,适配体结合表面分子印迹有望成为一个很好选择性提取材料。2、制备了一种双重识别的共振光传感器,用于检测双酚A。这种双重识别来源于适配体和表面分子印迹的共同作用。试验过程中考察了不同条件(pH,时间,粒子用量,温度等)对共振光响应值的影响。测试了它的选择性和相对标准偏差等。这种以纳米硅材料为主体交联适配体的分子印迹共振光传感器,制备过程简单经济,对检测双酚有一定的参考价值。3、以双酚A为分析对象,在适宜的管状材料上,通过电聚合方法制备结构和厚度可控的、具有高特异选择性的表面分子印迹聚合物膜。研究电聚合过程各因素对分子印迹聚合物结构以及性能的影响,探讨其构效关系。为后续将该表面分子印迹管连接到高效液相色谱仪上,构建管内固相微萃取—高效液相色谱联用分析系统,建立在线选择性分离富集环境水样中的BPA的方法做个铺垫。
[Abstract]:Bisphenol A, as an environmental estrogen, is very harmful to human beings and other living organisms. When bisphenol A enters into living organisms, it will interfere with the synthesis of normal endocrine substances, causing disorders in the body's major systems, resulting in a variety of diseases that endanger health, and even affect human reproduction. In recent years, in-tube solid-phase microextraction (SPME) for the separation and detection of trace bisphenol A (BPA) has been developed rapidly due to its advantages of automation, low cost and environmental friendliness. The polymer prepared by imprinting technique has certain selectivity to the target molecule and has been widely used in the separation and detection of bisphenol A. However, the surface molecularly imprinted polymer also has some nonspecific adsorption. As a highly specific recognition material, aptamer has been widely used in the detection of bisphenol A specificity. In this paper, combined with in-tube solid-phase microextraction, surface molecularly imprinted technique and high specific recognition ability of aptamer, the separation and detection of bisphenol A were studied, which provided a certain reference value for the separation and detection of bisphenol A. Molecularly imprinted polymers for specific recognition of bisphenol A were prepared. The adsorption and selectivity of the obtained aptamer-surface molecularly imprinted polymer microspheres were tested and compared with those of the conventional surface molecularly imprinted polymer microspheres. It was found that the aptamer-surface molecularly imprinted polymer microspheres improved the template efficiency. The molecular imprinting ability of the aptamer surface is twice that of the common surface molecule imprinting, and the aptamer in the imprinting hole has high selectivity to the target molecule. This indicates that the aptamer-binding surface molecule imprinting is expected to be a good selective extraction material. A dual-recognition resonant light sensor for detecting bisphenol A. The dual recognition results from the interaction of aptamer and surface molecular imprinting. The effects of different conditions (pH, time, particle dosage, temperature, etc.) on the resonant light response were investigated. The selectivity and relative standard deviation of the sensor were measured. Molecularly imprinted polymer films with controllable structure and thickness were prepared by electropolymerization on suitable tubular materials with bisphenol A as analytical object. The effects of various factors on the structure and properties of molecularly imprinted polymers (MIPs) during electropolymerization were studied, and their structure-activity relationships were discussed. Make a paving the way.
【学位授予单位】：湘潭大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：O631.3;X832

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