HPLC法测定5个采收期香蕉、野蕉、皇帝蕉果皮中羽扇豆酮
发布时间:2018-08-23 08:29
【摘要】:目的建立HPLC法测定3~7月采收的香蕉、野蕉、皇帝蕉果皮中羽扇豆酮的含有量。方法样品甲醇提取液的分析采用Diamonsil C18色谱柱(4.6 mm×250 mm,5μm);流动相乙腈-甲醇(1∶1);检测波长206 nm;体积流量1 m L/min;柱温25℃。结果羽扇豆酮在67.0~599.6μg/m L范围具有良好的线性关系(r=0.999 0),平均加样回收率98.57%,RSD 1.12%。3种果皮中该成分含有量有显著差异,依次为野蕉果皮香蕉果皮皇帝蕉果皮。不同采收期对香蕉果皮中其含有量也有明显影响,以4~7月较高,3月最低。结论建议以野蕉果皮或采收期在4~7月的香蕉果皮为羽扇豆酮主要来源。
[Abstract]:Objective to establish a HPLC method for the determination of lupinone in banana, banana and banana peel collected from March to July. Methods Diamonsil C18 column (4.6 mm 脳 250 mm ~ 5 渭 m); mobile phase acetonitrile-methanol (1:1), detection wavelength 206 nm, volumetric flow rate 1 mL / min, column temperature 25 鈩,
本文编号:2198489
[Abstract]:Objective to establish a HPLC method for the determination of lupinone in banana, banana and banana peel collected from March to July. Methods Diamonsil C18 column (4.6 mm 脳 250 mm ~ 5 渭 m); mobile phase acetonitrile-methanol (1:1), detection wavelength 206 nm, volumetric flow rate 1 mL / min, column temperature 25 鈩,
本文编号:2198489
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