制备色谱法分离纯化高良姜黄酮中高良姜素和山柰素
发布时间：2018-08-24 15:53
【摘要】：建立了制备型高效液相色谱(Prep-HPLC)分离高良姜黄酮中高良姜素和山柰素的方法。高良姜黄酮经HPD-600树脂吸附洗脱纯化后,采用Prep-HPLC分离高良姜黄酮中高良姜素和山柰素。制备色谱条件:流动相为甲醇-0.6%(v/v)乙酸水溶液(58∶42,v/v),柱温为常温,流速为7.0 mL/min,检测波长为360 nm,进样量为700μL,上样质量浓度为10.0 g/L。分离的单体由质谱和核磁共振氢谱、碳谱鉴定确证为高良姜素和山柰素,HPLC外标法定量,纯度分别为99.5%和99.7%。该方法分离效果好、高效、低毒,可用于高良姜中高良姜素和山柰素的分离制备。
[Abstract]:A method for the separation of galangin and kaempferol from flavonoids of alpinia officinalis by Prep-HPLC was established. The flavonoids of alpinia officinalis were eluted and purified by HPD-600 resin, and Prep-HPLC was used to separate alpinol and kaempferol from the flavonoids of alpinia officinalis. The chromatographic conditions were as follows: the mobile phase was methanol-0.6% (v / v) acetic acid solution (58: 42 v / v), the column temperature was normal temperature, the flow rate was 7.0 mL/min, the detection wavelength was 360 nm, the sample volume was 700 渭 L, and the sample concentration was 10.0 g / L. The isolated monomers were identified by mass spectrometry and nuclear magnetic resonance (NMR) and identified as gingerin and kaempferol by HPLC. The purity was 99.5% and 99.7%, respectively. The method has the advantages of good separation, high efficiency and low toxicity. It can be used for the separation and preparation of galangin and kaempferol from alpinia officinalis.
【作者单位】： 茂名职业技术学院;广东中轻枫泰生化科技有限公司;华南理工大学食品科学与工程学院;
【基金】：茂名市科技计划项目([2015]14)~~
【分类号】：O652.63;R284
本文编号：2201299
[Abstract]:A method for the separation of galangin and kaempferol from flavonoids of alpinia officinalis by Prep-HPLC was established. The flavonoids of alpinia officinalis were eluted and purified by HPD-600 resin, and Prep-HPLC was used to separate alpinol and kaempferol from the flavonoids of alpinia officinalis. The chromatographic conditions were as follows: the mobile phase was methanol-0.6% (v / v) acetic acid solution (58: 42 v / v), the column temperature was normal temperature, the flow rate was 7.0 mL/min, the detection wavelength was 360 nm, the sample volume was 700 渭 L, and the sample concentration was 10.0 g / L. The isolated monomers were identified by mass spectrometry and nuclear magnetic resonance (NMR) and identified as gingerin and kaempferol by HPLC. The purity was 99.5% and 99.7%, respectively. The method has the advantages of good separation, high efficiency and low toxicity. It can be used for the separation and preparation of galangin and kaempferol from alpinia officinalis.
【作者单位】： 茂名职业技术学院;广东中轻枫泰生化科技有限公司;华南理工大学食品科学与工程学院;
【基金】：茂名市科技计划项目([2015]14)~~
【分类号】：O652.63;R284
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