基于单细胞拉曼光谱技术的葡萄球菌黄素生物合成分析

发布时间：2018-08-31 11:59

【摘要】：利用光镊拉曼光谱技术研究吲哚对金葡菌细胞中葡萄球菌黄素合成的抑制作用以及色素含量在分批培养过程中的动态变化。收集经不同浓度吲哚(终浓度为0,0.2,0.6,0.8,1.2和1.5 mmol/L)处理后的以及不同培养时间的金葡菌单细胞的拉曼光谱,以光谱1523 cm-1峰强度表征色素含量,并与紫外可见分光光度法得到的结果进行比较。结果表明,细菌拉曼光谱1523 cm~(-1)峰强度与分光光度法测得的色素含量有良好的线性关系,相关系数达0.9772;群体和单细胞水平的光谱数据均表明,吲哚可剂量依赖性地抑制葡萄球菌黄素的合成,色素含量降低幅度超过70%;在分批培养中细菌色素含量在对数生长中期(12 h)达到最大值,各个时间点的群体内部细胞间色素含量的异质性较小,RSD在39.2%~61.1%之间。本研究表明光镊拉曼光谱技术是一种在单细胞水平分析葡萄球菌黄素含量的可靠方法。
[Abstract]:The inhibitory effect of indole on staphylococcal flavin synthesis in Staphylococcus aureus cells and the dynamic changes of pigment content in batch culture were studied by optical tweezers Raman spectroscopy. The Raman spectra of single cell of Staphylococcus aureus treated with different concentrations of indole (0 0. 2 and 0. 6 mmol/L) and cultured for different time were collected. The pigment content was characterized by the intensity of spectrum 1523 cm-1 peak. The results obtained by UV-vis spectrophotometry were compared with those obtained by UV-vis spectrophotometry. The results showed that there was a good linear relationship between the intensity of 1523 cm~ (-1) peak of bacterial Raman spectrum and the pigment content measured by spectrophotometry, and the correlation coefficient was 0.9772. Indole inhibited the synthesis of staphyloflavin in a dose-dependent manner, and the content of pigment decreased by more than 70%, and the content of bacterial pigment reached the maximum in the middle phase of logarithmic growth (12 h) in batch culture. The relative standard deviation (RSD) of cytochrome content between populations at different time points was between 39.2% and 61.1%. This study shows that optical tweezers Raman spectroscopy is a reliable method for the analysis of staphylococcal flavin at single cell level.
【作者单位】：广西科学院生物物理实验室;广西师范大学物理科学与技术学院;广西科学院国家非粮生物质能源工程技术研究中心;
【基金】：广西自然科学基金(No.2014GXNSFAA118193) 广西科学院基本科研业务费资助项目(No.15YJ22SL08)资助
【分类号】：O657.37

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