碳量子点的制备及其荧光特性研究
发布时间：2018-09-05 06:22
【摘要】：碳量子点(carbon quantum dots, CDs)是2004年发现的尺寸小于20 nm的新型纳米材料。碳量了点荧光明亮、稳定性高、无光漂白现象,发射波长可通过改变材料的粒径大小、结构成分或掺杂种类来调控,并具有良好的生物相容性和精确的生物靶向性以及低毒性,因而成为传统半导体量子点在生物成像、生物标记等应用中理想的替代物。由于碳量子点制备工艺简单、生产成木低廉,易于功能化和工业化,使其在环境检测、光催化、光电子器件等众多领域中展现出巨大的应用潜力,给生物医学、光电器件、薄膜显示等学科领域带来新的、广阔的发展空间。本文以新鲜柠檬汁/橙汁为碳源,采用水热法制备碳量子点,对碳量子点的表面形态、微观结构、结晶状态及荧光特性进行表征和分析,探索碳量子点的发光机制和影响因素：并利用碳量子点生物相容性和低细胞毒性以及优异的荧光特性实现了生物成像和肺腺癌细胞标记。研究结果表明：1.柠檬汁/橙汁水热法制备荧光碳量子点的最佳工艺参数为120℃下恒温150 min。此条件下制备的碳量子点粒径分布均匀,分散独立,结晶度高,稳定性好,能长时间稳定存在于水溶液中。2.柠檬汁/橙汁碳化合成碳量子点的原理是：柠檬汁/橙汁中的主要成分抗坏血酸、柠檬酸及糖类的碳化形成碳量子点。延长水热反应的时间会引起碳量子点团聚、变形和粒径分布不均等现象。3.用最佳工艺制备的碳量子点晶粒尺寸分布为2～4 nm,平均尺寸约为3 nm,晶格条纹清晰；对紫外光吸收较强,吸收峰位于202 nm和290 nm处；此方法制备的碳量子点表面存在大量含氧官能团,使之易溶于水,并有利于碳量子点表面改性和功能化。4.碳量子点的荧光波长具有激发光波长依赖的特性,随着激发光波长的增加,其荧光发射峰会发生小范围的红移,最强的荧光发射峰位于482nm处,呈蓝绿色荧光。将所制备荧光碳量子点用于肺腺癌细胞标记,用生物显微镜可观察到在蓝光激发下,碳量子点标记的肺腺癌细胞呈清晰的绿色荧光图像,细胞轮廓与白光明场像一致。
[Abstract]:Carbon quantum dot (carbon quantum dots, CDs) is a new nanometer material with size less than 20 nm discovered in 2004. Carbon content is bright, stable, photobleaching phenomenon, emission wavelength can be controlled by changing the particle size, structural composition or doping type of materials, and has good biocompatibility, accurate biological targeting and low toxicity. Therefore, it has become an ideal substitute for traditional semiconductor quantum dots in biological imaging, biomarker and other applications. Because of its simple preparation process, cheap wood production, easy functionalization and industrialization, carbon quantum dots have shown great application potential in many fields, such as environmental detection, photocatalysis, optoelectronic devices, and so on. Film display and other disciplines bring new and broad space for development. Using fresh lemon juice / orange juice as carbon source, carbon quantum dots were prepared by hydrothermal method. The surface morphology, microstructure, crystalline state and fluorescence characteristics of carbon quantum dots were characterized and analyzed. To explore the luminescence mechanism and influencing factors of carbon quantum dots (QDs), biocompatibility, low cytotoxicity and excellent fluorescence characteristics of QDs were used to achieve biologic imaging and labeling of lung adenocarcinoma cells. The results of the study show that 1: 1. Preparation of fluorescent carbon Quantum Dots by hydrothermal method of Lemon Juice / Orange Juice: 150 min. at constant temperature of 120 鈩，
本文编号：2223452
[Abstract]:Carbon quantum dot (carbon quantum dots, CDs) is a new nanometer material with size less than 20 nm discovered in 2004. Carbon content is bright, stable, photobleaching phenomenon, emission wavelength can be controlled by changing the particle size, structural composition or doping type of materials, and has good biocompatibility, accurate biological targeting and low toxicity. Therefore, it has become an ideal substitute for traditional semiconductor quantum dots in biological imaging, biomarker and other applications. Because of its simple preparation process, cheap wood production, easy functionalization and industrialization, carbon quantum dots have shown great application potential in many fields, such as environmental detection, photocatalysis, optoelectronic devices, and so on. Film display and other disciplines bring new and broad space for development. Using fresh lemon juice / orange juice as carbon source, carbon quantum dots were prepared by hydrothermal method. The surface morphology, microstructure, crystalline state and fluorescence characteristics of carbon quantum dots were characterized and analyzed. To explore the luminescence mechanism and influencing factors of carbon quantum dots (QDs), biocompatibility, low cytotoxicity and excellent fluorescence characteristics of QDs were used to achieve biologic imaging and labeling of lung adenocarcinoma cells. The results of the study show that 1: 1. Preparation of fluorescent carbon Quantum Dots by hydrothermal method of Lemon Juice / Orange Juice: 150 min. at constant temperature of 120 鈩，
本文编号：2223452
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