恩诺沙星分子印迹聚合物膜的制备及吸附性能研究

发布时间：2018-09-08 20:56

【摘要】：采用表面印迹法,以恩诺沙星为模板,甲基丙烯酸(MAA)为功能单体,乙二醇二甲基丙烯酸酯(EDMA)为交联剂,在聚苯乙烯酶标板表面直接合成恩诺沙星分子印迹聚合物膜。通过傅立叶红外光谱分析、电镜扫描、吸附平衡结合实验、Scatchard方程分析及吸附动力学实验对恩诺沙星印迹聚合物膜进行性能表征。合成的分子印迹聚合物膜具有很好的印迹效果,对恩诺沙星有较高的特异性吸附,且传质速率快,由Scatchard方程分析可知,聚合物膜中含有两类吸附位点,其中高亲和力位点的平衡解离常数(Kd)为19.49μg/mL,饱和吸附容量(Qmax)为12.98μg/mL,低亲和力位点的Kd为277.78μg/mL,Qmax为98.14μg/mL,吸附位点的异质性并不会影响聚合物膜应用于竞争性免疫吸附分析。通过该方法合成的恩诺沙星特异性识别聚合物膜可以作为仿生抗体,应用于竞争性免疫吸附分析检测恩诺沙星在食品中的残留。
[Abstract]:Using enrofloxacin as template, (MAA) as functional monomer and ethylene glycol dimethacrylate (EDMA) as crosslinking agent, enrofloxacin molecularly imprinted polymer membranes were synthesized directly on the surface of polystyrene enzyme labeled plate by surface imprinting. The properties of enrofloxacin imprinted polymer membranes were characterized by Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), adsorption equilibrium binding analysis (Scatchard equation) and adsorption kinetics experiments. The synthesized molecularly imprinted polymer membrane has a good imprinting effect, and has a high specific adsorption rate for enrofloxacin, and the mass transfer rate is fast. According to Scatchard equation analysis, the polymer membrane contains two kinds of adsorption sites. The equilibrium dissociation constant of high affinity sites is 19.49 渭 g / mL, the saturated adsorption capacity (Qmax) is 12.98 渭 g / mL, the Kd of low affinity sites is 277.78 渭 g / mL and the Qmax of low affinity sites is 98.14 渭 g / mL. The heterogeneity of adsorption sites will not affect the application of polymer membranes to competitive immunosorbent assay. The enrofloxacin specific recognition polymer membrane synthesized by this method can be used as bionic antibody to detect enrofloxacin residues in food by competitive immunosorbent assay.
【作者单位】：天津现代职业技术学院生物工程学院;天津科技大学食品工程与生物技术学院;
【分类号】：O631.3;TS207.5

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