胶体金核壳型沙丁胺醇分子印迹聚合物的光谱特征分析

发布时间：2018-09-09 19:59

【摘要】：为了解决饲料和动物产品中沙丁胺醇残留现场快速检测的难题,开发以分子印迹技术为基础的快速检测沙丁胺醇的新方法,使用沙丁胺醇做为模板分子,甲基丙烯酸(methacrylic acid,MA)作为功能单体,以本体聚合法为基础合成常规SAL分子印迹聚合物(molecularly imprinted polymer,MIPs)和非分子印迹聚合物(non imprinted polymer NIPs)。在此基础上,以胶体金粒子为引发核,制备出新型的核壳型沙丁胺醇MIPs。应用紫外吸收光谱(UV spectra)、傅里叶红外光谱(IR spectra)和拉曼光谱(Raman spectru)、扫描电镜(scanning electron microscopy,SEM)等技术手段获得两种印迹物及各种相关化合物的光谱图、电镜图等表征图像。由实验结果可知,SAL和MA上的羧基形成稳定又容易洗脱的1∶1型氢键配合物,化学结合常数K=-0.245×10~6 L~2·mol~(-2)。与MA的—COOH中氢原子形成氢键的可能结合位点是SAL C—O中的氧原子。MIPs与MA中—OH的吸收峰比较可知,前者明显红移;证明SAL作为模板分子与MA之间发生特定结合。未洗脱MIPs的C—O的伸缩振动产生的吸收峰红移;即能量损失明显,可知MA中—COOH的氢原子如果要生成氢键,可能的结合位点就是SAL分子内C—O中的氧原子。MIPs和NIPs中C—C,C—O,—OH等吸收明显的官能团峰型大致相同。将MIPs洗脱掉作为模板分子的SAL后,留下了含有特殊且确定结构官能团化学及空间构成均与SAL高度匹配的空穴,可与待测液中的目标检测分子SAL发生特异性识别和专一结合作用。而胶体金核壳型MIPs与常规MIPs相比,除具有以上相同特点外,其表面更加松散,表面孔穴明显增多。由此增加了吸附目标分子的有效面积,具有更优良的吸附性能。这两种印迹物的合成及光谱特征分析为建立基于分子印迹技术的快速检测SAL新方法奠定了理论和实践基础。
[Abstract]:In order to solve the problem of rapid detection of salbutamol residues in feed and animal products, a new method for rapid detection of salbutamol was developed based on molecular imprinting technology. Salbutamol was used as template molecule. Conventional SAL molecularly imprinted polymer (molecularly imprinted polymer,MIPs) and non-molecularly imprinted polymer (non imprinted polymer NIPs).) were synthesized by bulk polymerization of methacrylic acid (methacrylic acid,MA) as functional monomer. On this basis, a novel core-shell salbutamol MIPs. was prepared by using colloidal gold particles as initiator. UV absorption spectrum (UV spectra), Fourier transform infrared spectroscopy (IR spectra) and Raman spectrum (Raman spectru), scanning electron microscope (scanning electron microscopy,SEM) were used to obtain the spectra of two kinds of imprinted materials and their related compounds. From the experimental results, it can be seen that the carboxyl groups on the Sal and MA form a stable and easily eluted 1:1 hydrogen bond complex, and the chemical binding constant is K ~ (-0.245) 脳 10 ~ (6) L ~ (2) mol~ (-2). The possible binding sites to hydrogen atoms in MA's -COOH are the oxygen atoms in SAL C-O. MIPs and the absorption peaks of -OH in MA. It is shown that the former has a red shift, and it is proved that there is a specific binding between SAL as a template molecule and MA. The redshift of absorption peak caused by the stretching vibration of C-O of MIPs is not eluted, that is, the energy loss is obvious, it is known that if the hydrogen atom of -COOH in MA is to form hydrogen bond, The possible binding sites are the oxygen atoms. MIPs in C-O in SAL molecule and the apparent functional group peaks in NIPs. After removing the SAL as a template molecule, the MIPs wash left a hole with special structure functional group chemistry and space composition, which is highly matched with SAL, which can be specifically recognized and specifically combined with the target detection molecule SAL in the solution under test. Compared with conventional MIPs, colloidal gold core-shell MIPs has more loose surface and more porous surface. As a result, the effective area of the target molecule is increased and the adsorption performance is improved. The synthesis and spectral characteristic analysis of the two imprinted materials lay a theoretical and practical foundation for the establishment of a new method for rapid detection of SAL based on molecular imprinting.
【作者单位】：上海交通大学农业与生物学院;
【基金】：国家自然科学基金项目(31372344)资助
【分类号】：O657.33;O631.3

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