高效液相色谱-示差折光率法测定林业生物质原料中的可溶性糖

发布时间：2018-10-05 10:22

【摘要】：建立了高效液相色谱测定林业生物质原料中可溶性糖的含量和种类的方法。前处理方法为样品称样量4~5g,蒸馏水热浸提1.0 h。色谱条件为:色谱柱(XBridge Amide NUCLEOSIL100-5 NH24.6×250 mm,3.5μm);流动相为V(乙腈):V(水)=83:17,加入0.1%(V/V)的三乙胺;柱温为30℃;流速1.0 m L/min;进样量20μL。在此条件下,葡萄糖、木糖、阿拉伯糖、果糖、甘露糖和蔗糖6种糖的质量浓度分别与峰面积呈良好的线性关系(R20.9978),检测限在3.26~4.30 mg/L之间;加标回收率在96.2%~103.4%之间。对毛竹竹材,油茶饼粕,橡胶树树干,虎榛子树干,胡杨树干,白桦根、枝、树干,马尾松针叶,银杏叶,毛白杨树干等实际样品进行测定时发现,林木生物质的种类、取样部位不同,所含有的可溶性糖种类及含量差异较大。该方法可用于测定林业生物质原料中可溶性糖含量。
[Abstract]:A high performance liquid chromatography (HPLC) method for the determination of soluble sugar in forestry biomass was established. The pretreatment method was as follows: the sample weight was 4g and the distilled water was heated for 1.0 h. The chromatographic conditions are as follows: the mobile phase of (XBridge Amide NUCLEOSIL100-5 NH24.6 脳 250 mm,3.5 渭 m); is V (acetonitrile): v (water) 83: 17, 0.1% (V / V) triethylamine is added, the column temperature is 30 鈩，

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