基于血红蛋白-纳米磷酸钬复合材料的过氧化氢生物传感器

发布时间：2018-10-09 12:55

【摘要】：以Ho2O3为反应物,采用水热法制备了纳米磷酸钬(n-Ho PO4),并利用场发射扫描电子显微镜(SEM)、能谱分析(EDS)对其进行形貌表征和元素组成分析。将n-Ho PO4和血红蛋白(Hb)复合材料修饰于裸玻碳电极(GCE)表面构建生物传感器,实现了对H2O2的电化学检测。采用循环伏安(CV)和电化学交流阻抗(EIS)技术对修饰电极进行表征,结果表明,Hb/n-Ho PO4/GCE对H2O2的还原具有良好的电化学催化效果;n-Ho PO4具有良好的导电性和生物相容性,促进了Hb与工作电极间的直接电子转移。研究了不同p H值和电化学扫速对修饰电极响应电流的影响。在优化实验条件下,此生物传感器对H2O2在50~1000μmol/L范围内表现出良好的线性关系,相关线性系数R=0.999,检出限为17μmol/L(S/N=3)。此生物传感器具有检测范围宽、稳定性和重现性好、抗干扰能力强等优点,可用于实际样品的检测。
[Abstract]:Nanocrystalline holmium phosphate (n-Ho PO4) was prepared by hydrothermal method using Ho2O3 as reactant. The morphology and elemental composition of n-Ho PO4 were characterized by (EDS) with field emission scanning electron microscopy (SEM),). N-Ho PO4 and hemoglobin (Hb) composites were modified on the surface of bare glassy carbon electrode (GCE) to construct a biosensor for the electrochemical detection of H2O2. The modified electrode was characterized by cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS). The results showed that Hb / n-Ho PO4/GCE had a good electrochemical catalytic effect on the reduction of H2O2 and n-Ho PO4 had good conductivity and biocompatibility. The direct electron transfer between Hb and working electrode is promoted. The effects of pH value and electrochemical sweep speed on the response current of the modified electrode were studied. Under the optimized experimental conditions, the biosensor showed a good linear relationship with H2O2 in the range of 50 ~ 1000 渭 mol/L. The correlation coefficient was 0.999, and the detection limit was 17 渭 mol/L (S/N=3). The biosensor has the advantages of wide detection range, good stability and reproducibility, strong anti-interference ability, and can be used for the detection of practical samples.
【作者单位】：江西理工大学冶金与化学工程学院;
【基金】：国家自然科学基金(Nos.21465013,21005034) 中国博士后科学基金(No.2014M551550) 江西省自然科学基金(No.20114BAB213014) 江西理工大学清江青年英才支持计划资助项目~~
【分类号】：O657.1

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