火把花根片中间体的HPLC指纹图谱研究
发布时间：2018-10-09 15:07
【摘要】：目的:建立火把花根片中间体的高效液相色谱(HPLC)指纹图谱。方法:采用HPLC法。色谱柱为用Inertsil ODS-4,流动相为乙腈-0.1%磷酸溶液(梯度洗脱),流速为0.75 mL/min,检测波长为220 nm,柱温为30℃,进样量为10μL。以雷公藤晋碱为参照,测定10批样品的HPLC图谱,采用《中药色谱指纹图谱相似度评价系统》(2004 A版)进行共有峰指认和相似度评价。结果:10批火把花根片中间体的HPLC图谱有25个共有峰,相似度均0.90。经验证,10批样品HPLC图谱与对照指纹图谱具有较好的一致性。结论:该研究所建指纹图谱可为火把花根片中间体的鉴别和质量评价提供参考。
[Abstract]:Objective: to establish the (HPLC) fingerprint of the intermediate of Huanghuatou root tablets by high performance liquid chromatography (HPLC). Methods: HPLC method was used. The chromatographic column consisted of Inertsil ODS-4, mobile phase with acetonitrile-0.1% phosphoric acid solution (gradient elution), the flow rate was 0.75 mL/min, the detection wavelength was 220 nm, the temperature was 30 鈩，
本文编号：2259786
[Abstract]:Objective: to establish the (HPLC) fingerprint of the intermediate of Huanghuatou root tablets by high performance liquid chromatography (HPLC). Methods: HPLC method was used. The chromatographic column consisted of Inertsil ODS-4, mobile phase with acetonitrile-0.1% phosphoric acid solution (gradient elution), the flow rate was 0.75 mL/min, the detection wavelength was 220 nm, the temperature was 30 鈩，
本文编号：2259786
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